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===Homo heidelbergensis===
===Homo heidelbergensis===
In 2013, the mtDNA sequence from the femur of a 400,000 year old ''[[Homo heidelbergensis]]'' from the [[Atapuerca Mountains#Sima de los Huesos .281983-.29|Sima de los Huesos Cave]] in Spain was found to be most similar to that of Denisova<ref name=Callaway>{{cite journal|last=Callaway|first=Ewen|title=Hominin DNA baffles experts|journal=Nature|date=4 December 2013|volume=504|issue=7478|pages=16–17|doi=10.1038/504016a|url=http://www.nature.com/news/hominin-dna-baffles-experts-1.14294|accessdate=22 April 2014}}</ref>.
In 2013, the femur of a ~400,000 year old ''[[Homo heidelbergensis]]'' from the [[Atapuerca Mountains#Sima de los Huesos .281983-.29|Sima de los Huesos Cave]] in Spain was found to contain both mtDNA and nuclear DNA. Improvements in DNA extraction and library preparation techniques allowed for mtDNA to be successfully isolated and sequenced, however the nuclear DNA was found to be too degraded in the observed specimen, and was also contaminated with DNA from an ancient cave bear ('''Ursus deningeri''') also estimated to have lived ~400,000 years ago. The mtDNA analysis found a surprising link between the specimen and the Denisovans, and this finding raised many questions. Several scenarios were proposed in a January 2014 paper titled "A mitochondrial genome sequence of a hominin from Sima de los Huesos", elucidating the lack of convergence in the scientific community on how '''Homo heidelbergensis''' is related to other known hominin groups. One plausible scenario that the authors proposed was that the '''H. heidelbergensis''' an ancestor to both Denisovans and Neanderthals.<ref name=Meyer>{{cite journal|last=Meyer|first=Matthias|coauthors=Fu, Qiaomei; Aximu-Petri, Ayinuer; Glocke, Isabelle; Nickel, Birgit; Arsuaga, Juan-Luis; Martínez, Ignacio; Gracia, Ana; de Castro, José María Bermúdez; Carbonell, Eudald; Pääbo, Svante|title=A mitochondrial genome sequence of a hominin from Sima de los Huesos|journal=Nature|date=4 December 2013|volume=505|issue=7483|pages=403–406|doi=10.1038/nature12788|url=http://email.eva.mpg.de/~paabo/pdf1/Meyer_Mitochondrial_nature_2014.pdf|accessdate=23 April 2014}}</ref> Completely sequenced nuclear genomes from both Denisovans and Neanderthals suggest a common ancestor approximately 700,000 years ago, and one leading researcher in the field, Svante Paabo, suggests that perhaps this new hominin group is that early ancestor.<ref name=Callaway>{{cite journal|last=Callaway|first=Ewen|title=Hominin DNA baffles experts|journal=Nature|date=4 December 2013|volume=504|issue=7478|pages=16–17|doi=10.1038/504016a|url=http://www.nature.com/news/hominin-dna-baffles-experts-1.14294|accessdate=22 April 2014}}</ref>


==Applications==
==Applications==

Revision as of 05:15, 23 April 2014

Molecular paleontology refers to the recovery and analysis of DNA, proteins, carbohydrates, or lipids, and their diagenetic products from ancient human, animal, and plant remains.[1][2] The field of molecular paleontology has yielded important insights into evolutionary events, species' diasporas, the discovery and characterization of extinct species. By applying molecular analytical techniques to DNA in fossils, one can quantify the level of relatedness between any two organisms for which DNA has been recovered.[3]

Advancements in the field of Molecular Paleontology has allowed scientists to pursue evolutionary questions on a genetic level rather than relying on phenotypic variation alone. Using various biotechnological techniques such as DNA sequencing, isolation, and amplification,[4] scientists have been able to gain expanded new insights into the divergence and evolutionary history of countless organisms.

Extreme care must be taken to avoid human DNA contamination.

History

Timeline

A timeline demonstrating important dates in Molecular paleontology. All of these dates are listed and specifically sourced in the History section under Timeline.[1][5][6][7][8][9][10][11][12][13]

mid-1950s: Abelson found preserved amino acids in fossil shells that were about 360 million years old. Produced idea of comparing fossil amino acid sequences with existing organism so that molecular evolution could be studied.[5]

1970s: Fossil peptides are studied by amino acid analysis.[6] Start to use whole peptides and immunological methods.[7]

Late 1970s: Palaeobotanists studied molecules from well-preserved fossil plants.[8]

1984: The first successful DNA sequencing of an extinct species, the Quagga, a zebra-like species.[1]

1991: Published article on the successful extraction of proteins from the fossil bone of a dinosaur, specifically the seismosaurus.[9]

2005: Scientists resurrect extinct 1918 influenza virus.[10]

2007: Scientists synthesize entire extinct human endogenous retrovirus (HERV-K) from scratch.[11]

2010: A new species of early hominid, the Denisovans, discovered from mitochondrial and nuclear genomes recovered from bone found in a cave in Siberia. Analysis showed that the Denisovan specimen lived approximately 41,000 years ago, and shared a common ancestor with both modern humans and Neanderthals approximately 1 million years ago in Africa.[12]

2013: The first entire Neanderthal genome is successfully sequenced.[13]

2013 A 400,000-year-old specimen with remnant mitochondrial DNA sequenced and is found to be a common ancestor to Neanderthals and Denisovans, later named Homo heidelbergensis. [14].

===The Quagga=== The first successful DNA sequencing of an extinct species was in 1984, from a 150-year-old museum specimen of the quagga, a zebra-like species.[1] Mitochondrial DNA (also known as mtDNA) was sequenced from desiccated muscle of the quagga, and was found to differ by 12 base substitutions from the mitochondrial DNA of a mountain zebra. It was concluded that these two species had a common ancestor 3-4 million years ago, which is consistent with known fossil evidence of the species.[15]

Denisovans

The Denisovans of Eurasia, a hominid species related to Neanderthals and humans, was discovered as a direct result of DNA sequencing of a 41,000 year old specimen recovered in 2008. Analysis of the mitochondrial DNA from a retrieved finger bone showed the specimen to be genetically distinct from both humans and Neanderthals. Two teeth and a toe bone were later found to belong to different individuals with the same population. Analysis suggests that both the Neanderthals and Denisovans were already present throughout Eurasia when modern humans arrived.[13]

Mitochondrial DNA analysis

The mtDNA from the Denisovan finger bone differs from that of modern humans by 385 bases (nucleotides) in the mtDNA strand out of approximately 16,500, whereas the difference between modern humans and Neanderthals is around 202 bases. In contrast, the difference between chimpanzees and modern humans is approximately 1,462 mtDNA base pairs[16] . This suggested a divergence time around one million years ago. The mtDNA from a tooth bore a high similarity to that of the finger bone, indicating they belonged to the same population[17] . From a second tooth, an mtDNA sequence was recovered that showed an unexpectedly large number of genetic differences compared to that found in the other tooth and the finger, suggesting a high degree of mtDNA diversity. These two individuals from the same cave showed more diversity than seen among sampled Neanderthals from all of Eurasia, and were as different as modern-day humans from different continents[18] .

Nuclear genome analysis

Isolation and sequencing of nuclear DNA has also been accomplished from the Denisova finger bone. This specimen showed an unusual degree of DNA preservation and low level of contamination. They were able to achieve near-complete genomic sequencing, allowing a detailed comparison with Neanderthal and modern humans. From this analysis, they concluded, in spite of the apparent divergence of their mitochondrial sequence, the Denisova population along with Neanderthal shared a common branch from the lineage leading to modern African humans. The estimated average time of divergence between Denisovan and Neanderthal sequences is 640,000 years ago, and the time between both of these and the sequences of modern Africans is 804,000 years ago. They suggest the divergence of the Denisova mtDNA results either from the persistence of a lineage purged from the other branches of humanity through genetic drift or else an introgression from an older hominin lineage[17].

Homo heidelbergensis

In 2013, the femur of a ~400,000 year old Homo heidelbergensis from the Sima de los Huesos Cave in Spain was found to contain both mtDNA and nuclear DNA. Improvements in DNA extraction and library preparation techniques allowed for mtDNA to be successfully isolated and sequenced, however the nuclear DNA was found to be too degraded in the observed specimen, and was also contaminated with DNA from an ancient cave bear (Ursus deningeri) also estimated to have lived ~400,000 years ago. The mtDNA analysis found a surprising link between the specimen and the Denisovans, and this finding raised many questions. Several scenarios were proposed in a January 2014 paper titled "A mitochondrial genome sequence of a hominin from Sima de los Huesos", elucidating the lack of convergence in the scientific community on how Homo heidelbergensis is related to other known hominin groups. One plausible scenario that the authors proposed was that the H. heidelbergensis an ancestor to both Denisovans and Neanderthals.[19] Completely sequenced nuclear genomes from both Denisovans and Neanderthals suggest a common ancestor approximately 700,000 years ago, and one leading researcher in the field, Svante Paabo, suggests that perhaps this new hominin group is that early ancestor.[20]

Applications

Discovery and Characterization of New Species

Molecular paleontology techniques applied to fossils have contributed to the discovery and characterization of several new species, including the Denisovans and Homo heidelbergensis. We have been able to better understand the path that humans took as they populated the earth, and what species were present during this diaspora.

Neanderthal DNA extraction. Working in a clean room, researchers at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, took extensive precautions to avoid contaminating Neanderthal DNA samples - extracted from bones like this one - with DNA from any other source, including modern humans. NHGRI researchers are part of the international team that sequenced the genome of the Neanderthal, Homo neanderthalensis.

De-extinction

The Pyrenean ibex was temporarily brought back from extinction in 1984.

It is now possible to revive extinct species using molecular paleontology techniques. This was first accomplished via cloning in 2003 with the Pyrenean ibex, a type of wild goat that went extinct in 2000. Nuclei from the Pyrenean ibex's cells were injected into goat eggs emptied of their own DNA, and implanted into surrogate goat mothers.[21] The offspring lived only seven minutes after birth, due to defects in its lungs. Other cloned animals have been observed to have similar lung defects.[22]

There are many species that have gone extinct as a direct result of human activity. Some examples include the dodo, the great auk, the Tasmanian tiger, the Chinese river dolphin, and the passenger pigeon. An extinct species can be revived by using allelic replacement[23] of a closely related species that is still living. By only having to replace a few genes within an organism, instead of having to build the extinct species' genome from scratch, it could be possible to bring back several species in this way, even Neanderthals.[24]

The ethics surrounding the re-introduction of extinct species are very controversial. Critics of bringing extinct species back to life contend that it would divert limited money and resources from protecting the world's current biodiversity problems.[25] With current extinction rates approximated to be 100 to 1,000 times the background extinction rate,[26] it is feared that a de-extinction program might lessen public concerns over the current mass extinction crisis, if it is believed that these species can simply be brought back to life. As the editors of a Scientific American article on de-extinction pose: Should we bring back the wooly mammoth only to let elephants go extinct in the meantime? [25] The main driving factor for the extinction of most species in this era (post 10,000 B.C.) is the loss of habitat, and temporarily bringing back an extinct species will not recreate the environment they once inhabited.[27]

Proponents of De-extinction, such as George Church, speak of many potential benefits. Reintroducing an extinct keystone species, such as the wooly mammoth, could help re-balance the ecosystems that once depended on them. Some extinct species could create broad benefits for the environments they once inhabited, if returned. For example, wooly mammoths may be able to slow the melting of the Russian and Arctic tundra in several ways such as eating dead grass so that new grass can grow and take root, and periodically breaking up the snow, subjecting the ground below to the arctic air. These techniques could also be used to reintroduce genetic diversity in a threatened species, or even introduce new genes and traits to allow the animals to compete better in a changing environment.[28]


Research and Technology

When a new potential specimen is found, scientists normally first analyze for cell and tissue preservation using histological techniques, and test the conditions for the survivability of DNA. They will then attempt to isolate a DNA sample using the technique described below, and conduct a PCR amplification of the DNA to increase the amount of DNA available for testing. This amplified DNA is then sequenced. Care is taken to verify that the sequence matches the phylogenetic traits of the organism.[1] When an organism dies, a technique called amino acid dating can be used to age the organism. It inspects the degree of racemization of aspartic acid, leucine, and alanine within the tissue. As time passes, the D/L ratio (where "D" and "L" are mirror images of each other) increase from 0 to 1.[29] In samples where the D/L ratio of aspartic acid is greater than 0.08, ancient DNA sequences can not be retrieved (as of 1996).[30]

Mitochondrial DNA vs. Nuclear DNA

Unlike nuclear DNA (left), mitochondrial DNA is only inherited from the maternal lineage (right).

Mitochondrial DNA (mtDNA) is separate from one's nuclear DNA. It is present in organelles called mitochondria in each cell. Unlike nuclear DNA, which is inherited from both parents and rearranged every generation, an exact copy of mitochondrial DNA gets passed down from mother to her sons and daughters. The benefits of performing DNA analysis with Mitochondrial DNA is that it has a far smaller mutation rate than nuclear DNA, making tracking lineages on the scale of tens of thousands of years much easier. Knowing the base mutation rate for mtDNA[31] , (in humans this rate is also known as the Human mitochondrial molecular clock) one can determine the amount of time any two lineages have been separated. Another advantage of mtDNA is that thousands of copies of it exist in every cell, whereas only two copies of nuclear DNA exist in each cell.[32] All eukaryotes, a group which includes all plants, animals, and fungi, have mtDNA.[33] A disadvantage of mtDNA is that only the maternal line is represented. For example, a child will inherit 1/8 of it's DNA from each of its eight great-grandparents, however it will inherit an exact clone of its maternal great-grandmother's mtDNA. This is analogous to a child inheriting only his paternal great-grandfather's last name, and not a mix of all of the eight surnames.



Isolation

There are many things to consider when isolating a substance. First, depending upon what it is and where it is located, there are protocols that must be carried out in order to avoid contamination and further degradation of the sample.[4] Then, handling of the materials is usually done in a physically isolated work area and under specific conditions (ie. specific Temperature, moisture, etc...) also to avoid contamination and further loss of sample.[4]

Once the material has been obtained, depending on what it is, there are different ways to isolate and purify it. DNA extraction from fossils is one of the more popular practices and there are different steps that can be taken to get the desired sample.[4] DNA extracted from amber-entombed fossils can be taken from small samples and mixed with different substances, centrifuged, incubated, and centrifuged again.[34] On the other hand, DNA extraction from insects can be done by grinding the sample, mixing it with buffer, and undergoing purification through glass fiber columns.[35] In the end, regardless of how the sample was isolated for these fossils, the DNA isolated must be able to undergo amplification.[35] [4] [34]

Amplification

Polymerase chain reaction

The field of molecular paleontology benefited greatly from the invention of the polymerase chain reaction(PCR), which allows one to make billions of copies of a DNA fragment from just a single preserved copy of the DNA. One of the biggest challenges up until this point was the extreme scarcity of recovered DNA because of degradation of the DNA over time.[1]

Sequencing

DNA sequencing is done to determine the order of nucleotides and genes.[36] There are many different materials from which DNA can be extracted from. In animals, the mitochondrial chromosome can be used for molecular study. Chloroplasts can be studied in plants as a primary source of sequence data.[36]

An evolutionary tree of mammals

In the end, the sequences generated are used to build evolutionary trees.[36] Methods to match data sets include: maximum probability, minimum evolution (also known as neighbor-joining) which searches for the tree with shortest overall length, and the maximum parsimony method which finds the tree requiring the fewest character-state changes.[36] The groups of species defined within a tree can also be later evaluated by statistical tests, such as the bootstrap method, to see if they are indeed significant.[36]

Limitations and Challenges

The largest challenge in extracting ancient DNA, particularly in ancient human DNA, is in contamination during PCR. Small amounts of human DNA can contaminate the reagents used for extraction and PCR of ancient DNA. These problems can be overcome by rigorous care in the handling of all solutions as well as the glassware and other tools used in the process. It can also help if only one person performs the extractions, to minimize different types of DNA present.[30]

Ideal environmental conditions for preserving DNA are where the organism was quickly desiccated and when uncovered is visually well-preserved. Nuclear DNA normally degrades rapidly after death by endogenous hydrolytic processes,[30] by UV radiation,[1] and other environmental stressors.

See also

References

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