On May 15, the new regiment traveled by train to Baltimore, Maryland, where it was assigned to the Second Separate Brigade of the VIII Corps. The 131st never saw any combat. Instead, it primarily served on garrison duty at Fort McHenry, then at Fort Marshall and Federal Hill. Detachments served at Washington, D.C., Harpers Ferry, West Virginia, Fortress Monroe, and City Point, Virginia. On August 19 the regiment was ordered to return to Ohio because the soldiers were nearing the end of their short term of enlistment. The 865 remaining men mustered out at Camp Chase on August 25, 1864. The 131st Regiment lost two enlisted men by disease during its service.
Ohio Roster Commission. Official Roster of the Soldiers of the State of Ohio in the War on the Rebellion, 1861–1865, compiles under the direction of the Roster commission. 12 vol. Akron: Werner Co., 1886–95.