2'-O-methylation is a common nucleoside modification of RNA, where a methyl group is added to the 2' hydroxyl group of the ribose moiety of a nucleoside. 2'-O-methylated nucleosides are mostly found in ribosomal RNA and small nuclear RNA and occur in the functionally essential regions of the ribosome and splicesome. Also, 2'-O-methylation of adenosine in an RNA prevents adenosine deaminase from editing it to an inosine.
Recently a novel method to map 2'-O ribose methylations by high throughput sequencing has been published.  The method is quantitative and maps all modifications in a single experiment.
- Kiss T (2001). "Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs". EMBO J. 20 (14): 3617–3622. doi:10.1093/emboj/20.14.3617. PMC 125535. PMID 11447102.
- Beal PA, Maydanovych O, Pokharel S (2007). "The chemistry and biology of RNA editing by adenosine deaminases". Nucleic Acids Symposium Series 51 (1): 83–84. doi:10.1093/nass/nrm042. PMID 18029597.
- Birkedal U et. al. (2015). "Profiling of Ribose Methylations in RNA by High-Throughput Sequencing". Angew. Chem. Int. Ed. 54 (2): 451–455. doi:10.1002/anie.201408362. PMID 25417815.
|This molecular biology article is a stub. You can help Wikipedia by expanding it.|