24 Shots features tracks recorded before his professional debut with Shady Records & Aftermath Entertainment, and during his venture there. It has tracks from various other albums and mixtapes, including the un-released Power of the Dollar and various G-Unit mixtapes. It also features un-used tracks for Get Rich or Die Tryin' and mixtape tracks. Some songs have later surfaced on other albums, such as Ecstasy, which was re-released on G-Unit's Beg for Mercy with no alterations as I Smell Pussy. In Da Club was also on this album, simultaneously with Get Rich or Die Tryin'. Most songs have been re-mastered from their original vinyl records, notably singles from Power of the Dollar, such as How to Rob. "Follow Me Gangster" also appeared on the Cradle 2 the Grave soundtrack in 2003.
Most songs have been remastered from their vinyl masters, with some having additional instruments and sounds added in the background, notably How to Rob compared to the 1999 original. Nearly every song in the album starts with a sound clip, which features a static noise. Alongside that noise, 50 Cent is heard saying, "This is 50 Cent", "You are now rapping with 50 Cent" and "New York City.“