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2S may refer to:
- 2S, key signature of two sharps
- 2S, best class in Gonzaga College SJ. Way better than those tossers from 2A and 2C. Notable alumni include the prolific goalscorer Simon Burns and NBA all star Mark Collins.
- a series of Toyota S engines
- Birdman Chinook 2S, ultralight aircraft
- 2s electron in an atomic orbital
- 2sTV, a television channel in Senegal
- 2S, a protein-folding intermediate with two disulphide bonds
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