|Jmol-3D images||Image 1|
|Molar mass||240.3482 g/mol|
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Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). TMB is a white crystal powder that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights.
The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at a wavelength of 650 nm.
TMB should be kept out of direct sunlight as it is photosensitive. It is not known if TMB is carcinogenic and the evidence is contradictory: TMB is not mutagenic by the Ames test, and did not cause tumours in 24 rats (but that experiment was uncontrolled). On that evidence, it has been used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.
- Sigma Aldrich Catalog Entry for 3,3′,5,5′-Tetramethylbenzidine
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