3,5-Dinitrosalicylic acid

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3,5-Dinitrosalicylic acid[1]
3,5-Dinitrosalicylic Acid Structural Formulae V.1.svg 3,5-dinitrosalicylic-acid-3D-balls.png
CAS number 609-99-4 N
ChemSpider 11380 YesY
KEGG C11319 YesY
ChEBI CHEBI:53648 YesY
Jmol-3D images Image 1
Molecular formula C7H4N2O7
Molar mass 228.12 g mol−1
Appearance yellow needles or plates
Melting point 182 °C (360 °F; 455 K)
Solubility in water soluble in water
Solubility soluble in ethanol, diethyl ether, benzene
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references

3,5-Dinitrosalicylic acid (DNS or DNSA, IUPAC name 2-hydroxy-3,5-dinitrobenzoic acid) is an aromatic compound that reacts with reducing sugars and other reducing molecules to form 3-amino-5-nitrosalicylic acid, which absorbs light strongly at 540 nm. It was first introduced as a method to detect reducing substances in urine and has since been widely used, for example, for quantifying carbohydrates levels in blood. It is mainly used in assay of alpha-amylase. However, enzymatic methods are usually preferred due to DNS lack of specificity.[2]

External links[edit]


  1. ^ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. pp. 3–318. ISBN 0-8493-0594-2. 
  2. ^ Miller, Gail Lorenz (1959). "Use of dinitrosalicylic acid reagent for determination of reducing sugar". Anal. Chem. 31 (3): 426–428. doi:10.1021/ac60147a030.