3,9-dihydroxypterocarpan 6a-monooxygenase

3,9-dihydroxypterocarpan 6a-monooxygenase
Identifiers
EC number	1.14.13.28
CAS number	92584-16-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

In enzymology, a 3,9-dihydroxypterocarpan 6a-monooxygenase (EC 1.14.13.28) is an enzyme that catalyzes the chemical reaction

(6aR,11aR)-3,9-dihydroxypterocarpan + NADPH + H⁺ + O₂ $\rightleftharpoons$ (6aS,11aS)-3,6a,9-trihydroxypterocarpan + NADP⁺ + H₂O

The 4 substrates of this enzyme are (6aR,11aR)-3,9-dihydroxypterocarpan, NADPH, H⁺, and O₂, whereas its 3 products are (6aS,11aS)-3,6a,9-trihydroxypterocarpan, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is (6aR,11aR)-3,9-dihydroxypterocarpan,NADPH:oxygen oxidoreductase (6a-hydroxylating). Other names in common use include 3,9-dihydroxypterocarpan 6a-hydroxylase, and 3,9-dihydroxypterocarpan 6alpha-monooxygenase (erroneous). This enzyme participates in isoflavonoid biosynthesis. It employs one cofactor, heme.

References

• Hagmann ML, Heller W, Grisebach H (1984). "Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures". Eur. J. Biochem. 142 (1): 127–31. doi:10.1111/j.1432-1033.1984.tb08259.x. PMID 6540173.