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35S or 35-S may refer to:
- Wasco State Airport's FAA airport code
- 35th parallel south, a geographical latitude
- Rollei 35S, a 35mm camera
- 35S, the most stable radioactive isotope of sulfur, used in biochemical research to radioactively label proteins
- HP 35s, a scientific calculator
- the 35S promoter from the cauliflower mosaic virus, used for constitutive expression in plants
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