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3T3-L1 is a cell line derived from (mouse) 3T3 cells that is used in biological research on adipose tissue. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype.

3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. These cells are also sensitive to lipogenic and lipolytic hormones and drugs, including epinephrine, isoproterenol, and insulin.[1] 3T3-L1 cells are extensively used to study adipogenesis.[2]


  1. ^ Green H, Kehinde O (1975). "An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion". Cell 5 (1): 19–27. doi:10.1016/0092-8674(75)90087-2. PMID 165899. 
  2. ^ Atanasov AG, Wang JN, Gu SP, Bu J, Kramer MP, Baumgartner L, Fakhrudin N, Ladurner A, Malainer C, Vuorinen A, Noha SM, Schwaiger S, Rollinger JM, Schuster D, Stuppner H, Dirsch VM, Heiss EH. Honokiol: A non-adipogenic PPARγ agonist from nature. Biochim Biophys Acta. 2013 Oct;1830(10):4813-9. doi:10.1016/j.bbagen.2013.06.021. Epub 2013 Jun 27. PubMed PMID: 23811337. http://www.ncbi.nlm.nih.gov/pubmed/23811337