ACES (buffer)
| ACES (buffer) | |
|---|---|
.png) |
|
|
2-(carbamoylmethylamino)ethanesulfonic acid |
|
|
Other names
N-(2-Acetamido)-2-aminoethanesulfonic acid |
|
| Identifiers | |
| CAS number | 7365-82-4 |
| PubChem | 81832 |
| ChemSpider | 73843  |
| ChEBI | CHEBI:39062 |
| Jmol-3D images | Image 1 Image 2 |
|
|
|
|
| Properties | |
| Molecular formula | C4H10N2O4S |
| Molar mass | 182.199 |
 (verify) (what is:  / ?)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
|
| Infobox references | |
ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid.
ACES is one of Good's buffers developed in the 1960s to provide buffers with pH ranging from 6.15-8.35 for use in various applications. With a pKa of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.[1]
[edit] Applications
ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.[2] ACES use in isoelectric focusing of proteins has also been documented.[3] Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE system.[4] Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.[5]
[edit] References
- ^ Good, N.E., "Hydrogen ion buffers for biological research." "Biochemistry", 5(2), 467-477.
- ^ Liu, Q., et al., "pK-matched running buffers for gel electrophoresis." "Anal. Biochemistry.", 270(1):112-122.
- ^ Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." "Electrophoresis", 9(2):65-73.
- ^ Strating, H., and Clarke, A.J., "Differentiation of bacterial autolysins by zymogram analysis." "Anal. Biochem.", 291(1):149-154.
- ^ Tunnicliff, G., and Smith, J.A., "Competitive inhibition of γ-aminobutyric acid receptor binding by N-2-hydroxyethylpiperazine-N'-2-ε-ethanesulfonic acid and related buffer." "J. Neurochem.", 36(3):1122-1126.
 |
This biochemistry article is a stub. You can help Wikipedia by expanding it. |
