ACF (gene)

From Wikipedia, the free encyclopedia
Jump to: navigation, search
APOBEC1 complementation factor
Protein ACF PDB 2cpd.png
PDB rendering based on 2cpd.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols A1CF ; ACF; ACF64; ACF65; APOBEC1CF; ASP
External IDs MGI1917115 HomoloGene16363 GeneCards: A1CF Gene
RNA expression pattern
PBB GE ACF 220951 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 29974 69865
Ensembl ENSG00000148584 ENSMUSG00000052595
UniProt Q9NQ94 Q5YD48
RefSeq (mRNA) NM_001198818 NM_001081074
RefSeq (protein) NP_001185747 NP_001074543
Location (UCSC) Chr 10:
52.57 – 52.65 Mb
Chr 19:
31.87 – 31.95 Mb
PubMed search [1] [2]

APOBEC1 complementation factor is a protein that in humans is encoded by the A1CF gene.[1][2][3]

Gene[edit]

Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined.[3]

Function[edit]

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC1 and a complementation factor encoded by this gene.[4] The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.[3]

Its deletion results in lethality in mice.[5]

Interactions[edit]

ACF has been shown to interact with APOBEC1,[6][7] , CUGBP2,[8] and SYNCRIP.[9][6]

References[edit]

  1. ^ Dance GS, Sowden MP, Cartegni L, Cooper E, Krainer AR, Smith HC (April 2002). "Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing". J Biol Chem 277 (15): 12703–9. doi:10.1074/jbc.M111337200. PMID 11815617. 
  2. ^ Chester A, Scott J, Anant S, Navaratnam N (December 2000). "RNA editing: cytidine to uridine conversion in apolipoprotein B mRNA". Biochim Biophys Acta 1494 (1-2): 1–13. doi:10.1016/S0167-4781(00)00219-0. PMID 11072063. 
  3. ^ a b c "Entrez Gene: ACF apobec-1 complementation factor". 
  4. ^ Henderson JO, Blanc V, Davidson NO (November 2001). "Isolation, characterization and developmental regulation of the human apobec-1 complementation factor (ACF) gene". Biochim. Biophys. Acta 1522 (1): 22–30. doi:10.1016/S0167-4781(01)00295-0. PMID 11718896. 
  5. ^ Blanc V, Henderson JO, Newberry EP, Kennedy S, Luo J, Davidson NO (August 2005). "Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality". Mol. Cell. Biol. 25 (16): 7260–9. doi:10.1128/MCB.25.16.7260-7269.2005. PMC 1190267. PMID 16055734. 
  6. ^ a b Blanc V, Navaratnam N, Henderson J O, Anant S, Kennedy S, Jarmuz A, Scott J, Davidson N O (March 2001). "Identification of GRY-RBP as an apolipoprotein B RNA-binding protein that interacts with both apobec-1 and apobec-1 complementation factor to modulate C to U editing". J. Biol. Chem. 276 (13): 10272–83. doi:10.1074/jbc.M006435200. PMID 11134005. 
  7. ^ Mehta A, Kinter M T, Sherman N E, Driscoll D M (March 2000). "Molecular cloning of apobec-1 complementation factor, a novel RNA-binding protein involved in the editing of apolipoprotein B mRNA". Mol. Cell. Biol. 20 (5): 1846–54. doi:10.1128/MCB.20.5.1846-1854.2000. PMC 85365. PMID 10669759. 
  8. ^ Anant S, Henderson J O, Mukhopadhyay D, Navaratnam N, Kennedy S, Min J, Davidson N O (December 2001). "Novel role for RNA-binding protein CUGBP2 in mammalian RNA editing. CUGBP2 modulates C to U editing of apolipoprotein B mRNA by interacting with apobec-1 and ACF, the apobec-1 complementation factor". J. Biol. Chem. 276 (50): 47338–51. doi:10.1074/jbc.M104911200. PMID 11577082. 
  9. ^ Lau PP, Chang B H, Chan L (April 2001). "Two-hybrid cloning identifies an RNA-binding protein, GRY-RBP, as a component of apobec-1 editosome". Biochem. Biophys. Res. Commun. 282 (4): 977–83. doi:10.1006/bbrc.2001.4679. PMID 11352648. 

Further reading[edit]