ADF-H domain

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PDB 1f7s EBI.jpg
crystal structure of adf1 from arabidopsis thaliana
Symbol Cofilin_ADF
Pfam PF00241
Pfam clan CL0092
InterPro IPR002108
SCOP 2prf
CDD cd00013

In molecular biology, ADF-H domain (actin-depolymerising factor homology domain) is an approximately 150 amino acid motif that is present in three phylogenetically distinct classes of eukaryotic actin-binding proteins.[1][2][3]

  • Twinfilins, which are actin monomer-binding proteins that are composed of two ADF-H domains
  • Abp1/Drebrins, which are relatively large proteins composed of an N-terminal ADF-H domain followed by a variable region and a C-terminal SH3 domain. Abp1/Drebrins interact only with actin filaments and do not promote filament depolymerisation or fragmentation. Although these proteins are biochemically distinct and play different roles in actin dynamics, they all appear to use the ADF-H domain for their interactions with actin.

The ADF-H domain consists of a six-stranded mixed beta-sheet in which the four central strands (beta2-beta5) are anti-parallel and the two edge strands (beta1 and beta6) run parallel with the neighbouring strands. The sheet is surrounded by two alpha-helices on each side .[1][2][4]


  1. ^ a b Lappalainen P, Kessels MM, Cope MJ, Drubin DG (August 1998). "The ADF homology (ADF-H) domain: a highly exploited actin-binding module". Mol. Biol. Cell 9 (8): 1951–9. doi:10.1091/mbc.9.8.1951. PMC 25446. PMID 9693358. 
  2. ^ a b Paavilainen VO, Merckel MC, Falck S, Ojala PJ, Pohl E, Wilmanns M, Lappalainen P (November 2002). "Structural conservation between the actin monomer-binding sites of twinfilin and actin-depolymerizing factor (ADF)/cofilin". J. Biol. Chem. 277 (45): 43089–95. doi:10.1074/jbc.M208225200. PMID 12207032. 
  3. ^ Liu LX, Xu H, Weller PF, Shi A, Debnath I (February 1997). "Structure and expression of a novel filarial gene for glia maturation factor". Gene 186 (1): 1–5. doi:10.1016/S0378-1119(96)00585-9. PMID 9047337. 
  4. ^ Liu L, Wei Z, Wang Y, Wan M, Cheng Z, Gong W (November 2004). "Crystal structure of human coactosin-like protein". J. Mol. Biol. 344 (2): 317–23. doi:10.1016/j.jmb.2004.09.036. PMID 15522287. 

This article incorporates text from the public domain Pfam and InterPro IPR002108