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AFC is an abbreviation that may refer to:
- Application Foundation Classes, a graphical framework for building Java-based graphical user interfaces (GUIs), developed by Microsoft
- Automated Fare Collection System
- Automatic frequency control
- Alkaline fuel cell, a type of fuel cell
- Aggression Fighting Championship, formerly Aggression MMA and Armageddon Fighting Championship a Canadian mixed martial arts promotion
- Asian Football Confederation, the governing body of association football in Asia
- American Football Conference, one of the two conferences of the National Football League (NFL) originally American Football League (AFL) (gridiron football)
- Aberdeen F.C., a professional association football club based in Aberdeen, Scotland
- Adelaide Football Club, a professional Australian rules football club based in Adelaide, South Australia, Australia
- AFC Ajax, a professional association football club based in Amsterdam, Netherlands
- Airdrieonians F.C., a professional association football club based in Airdrie, Scotland
- Amsterdamsche FC, a professional association football club based in Amsterdam, Netherlands
- Arsenal F.C., a professional association football club based in North London, England
- AFC Wimbledon, a professional association football club owned by the fans. Origins in Wimbledon, but is based in Kingston upon Thames, London, England.
- AFC Enterprises, the franchisor of Popeye's Chicken and Biscuits
- Agenda for Change, the current NHS pay grade system
- Alabama Forestry Commission
- America First Committee, historical US non-interventionist group
- Army Foundation College, British future soldier training organization
- Association Française des directeurs de la photographie Cinématographique, the French Society of Cinematographers
- Australian Film Commission, Australian government's film industry promotion commission
- Australian Flying Corps
- Asian Food Channel, a 24-hour Asian food TV channel in Asia.
- Antral follicle count, ovarian health statistic
- Average fixed cost, economic term
- Air Force Cross (United Kingdom), a British military decoration
- Air Force Cross (United States), an American military decoration
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