Actin-binding protein anillin is a protein that in humans is encoded by the ANLNgene. It is a 124 kDa scaffolding protein involved in cytokinesis. It is found in high concentrations near the cleavage furrow and coincides with RhoA, the key regulator of contractile-ring formation.
At the N-terminus, there is an actin and myosin binding domain; at the C terminus, there is a PH domain, which is conserved and is essential for its function. Anillin is highly enriched and abundant in metazoan cleavage furrows and provides an excellent cytological marker.
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