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Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Protein APPL1 PDB 2ela.png
PDB rendering based on 2ela.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols APPL1 ; APPL; DIP13alpha
External IDs OMIM604299 MGI1920243 HomoloGene32143 GeneCards: APPL1 Gene
RNA expression pattern
PBB GE APPL1 218158 s at tn.png
More reference expression data
Species Human Mouse
Entrez 26060 72993
Ensembl ENSG00000157500 ENSMUSG00000040760
UniProt Q9UKG1 Q8K3H0
RefSeq (mRNA) NM_012096 NM_145221
RefSeq (protein) NP_036228 NP_660256
Location (UCSC) Chr 3:
57.26 – 57.31 Mb
Chr 14:
26.92 – 26.97 Mb
PubMed search [1] [2]

DCC-interacting protein 13-alpha is a protein that in humans is encoded by the APPL1 gene.[1][2][3]

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.[3]

Mouse Mutant Alleles for Appl1
Marker Symbol for Mouse Gene. This symbol is assigned to the genomic locus by the MGI Appl1
Mutant Mouse Embryonic Stem Cell Clones. These are the known targeted mutations for this gene in a mouse. Appl1tm1a(KOMP)Wtsi
Example structure of targeted conditional mutant allele for this gene
Molecular structure of Appl1 region with inserted mutation sequence
These Mutant ES Cells can be studied directly or used to generate mice with this gene knocked out. Study of these mice can shed light on the function of Appl1:

see Knockout mouse


APPL1 has been shown to interact with Deleted in Colorectal Cancer[4] and AKT2.[1]


  1. ^ a b Mitsuuchi Y, Johnson SW, Sonoda G, Tanno S, Golemis EA, Testa JR (Oct 1999). "Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2". Oncogene 18 (35): 4891–8. doi:10.1038/sj.onc.1203080. PMID 10490823. 
  2. ^ Nechamen CA, Thomas RM, Dias JA (Nov 2006). "APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex". Mol Cell Endocrinol. 260-262: 93–9. doi:10.1016/j.mce.2006.08.014. PMC 1782224. PMID 17030088. 
  3. ^ a b "Entrez Gene: APPL1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1". 
  4. ^ Liu, Jiayou; Yao Fayi; Wu Ruping; Morgan Michael; Thorburn Andrew; Finley Russell L; Chen Yong Q (Jul 2002). "Mediation of the DCC apoptotic signal by DIP13 alpha". J. Biol. Chem. (United States) 277 (29): 26281–5. doi:10.1074/jbc.M204679200. ISSN 0021-9258. PMID 12011067. 

Further reading[edit]