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Agonist (from Ancient Greek ἀγωνιστής: agōnistēs, “combatant, champion”) may refer to:
- Agonist, A person engaged in a contest, conflict, struggle, etc. (see agon)
- Agonist, A person who is torn by inner conflict.
- An advocate of the political theory of agonism (or 'agonistic pluralism').
- Agonist, a substance that binds to a receptor to induce a biochemical response
- Inverse agonist, a substance that induces the opposite effect of an agonist
- Agonist (muscle), a muscle type
- Samson Agonistes, a tragedy by John Milton.
- The Agonist, a Canadian metal band
- Agonistic behaviour, animal behaviour relating to conflict
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