Aiserey is located some 20 km south-east of Dijon and 32 km north-east of Beaune. It can be accessed by Highway D968 from Dijon and Longecourt-en-Plaine in the north-west continuing through the heart of the commune and the village on to Brazey-en-Plaine in the south-east. There is also the D116 coming from Izeure in the west continuing to Echigey in the east. The railway line running south-east from Dijon passes through the commune and there is a station next to the village. In addition to the village there is the small village of Potangey in the north. The rest of the commune is entirely farmland apart from the former sugar refinery in the east.
The Canal de Bourgogne passes through the commune on the eastern side from north-west to south-east. The Oucherolle stream rises near the village and flows to the north-west. There are reservoirs in the east of the commune linked to the canal.
In 2009, the commune had 1,357 inhabitants. The evolution of the number of inhabitants is known through the population censuses conducted in the commune since 1793. From the 21st century, a census of communes with fewer than 10,000 inhabitants is held every five years, unlike larger towns that have a sample survey every year.[Note 1]
^At the beginning of the 21st century, the methods of identification have been modified by law No. 2002-276 of 27 February 2002 , the so-called "law of local democracy" and in particular Title V "census operations" which allow, after a transitional period running from 2004 to 2008, the annual publication of the legal population of the different French administrative districts. For municipalities with a population greater than 10,000 inhabitants, a sample survey is conducted annually, the entire territory of these municipalities is taken into account at the end of the period of five years. The first "legal population" after 1999 under this new law came into force in 1 January 2009 and was based on the census of 2006.