The museum includes Albin Polasek's former retirement home, studio, galleries, and chapel, as well as surrounding gardens containing figurative sculpture and mythological pieces. The permanent collection of approximately 200 pieces includes sculpture by Polasek and his first wife, Ruth Sherwood, as well as antiquities from Polasek's collection. The galleries feature changing exhibitions of local artists for viewing and for sale. Special areas to visit in the garden include the butterfly garden, two water gardens, the cycad collection along the museum’s front drive and the many individual container gardens found throughout the property.
Each April the Albin Polasek Museum & Sculpture Gardens hosts the Winter Park Paint Out, during which artists paint en plein air, French for "in the open air," throughout the streets of Winter Park. The artists will have seven days to publicly create their works, which are offered for sale as a fundraiser for the museum.