Bayerová was born near Mělník on 4 November 1853, the youngest child of Josef and Marie Bayer. She attended school in Mělník until 1868, when she moved to Prague, where she met the authors Eliška Krásnohorská and Sofie Podlipská, and took gymnasial exams, despite not being allowed to attend the gymnasium.
In 1875, Bayerová moved to Zurich and started attending classes at the University of Zurich. However, she suffered financial problems and returned home in 1878. Eventually, after receiving money to continue her studies, Anna Bayerová became the second female Czech physician in 1881.
She died on 24 January 1924 in Prague.
- Honzáková, Anna (1937): Doctor of Medicine: Anna Bayerová 1853–1924 (Prague)
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