In 1894 Žmuidzinavičius graduated from Veiveriai Teachers' Seminary. From 1899 studied at various European universities. Later Žmuidzinavičius delivered lectures about Lithuania. Together with Lithuanian painter and composer M.K. Čiurlionis and other prominent Lithuanians organized the First Exhibition of Lithuanian Art at Petras Vileišishouse. Between 1926 and 1940 he taught drawing in Kaunas, and in 1947 Žmuidzinavičius became a professor. Among his most famous paintings are Gavo laišką (Received a Letter, 1904), Paskutiniai spinduliai (Last Rays, 1908), Estijos peizažas (Landscape of Estonia, 1929).