Antigen presentation describes a vital process of the immune system. Immune cells cannot "see inside" other cells, which may be infected with viruses or bacteria, and thus rely on information conveyed by fragments of intracellular components being presented on the cell surface on Major histocompatibility complex (MHC) molecules. Likewise, many pathogens are too large to be recognised directly by immune cells, and must first be digested into smaller fragments that can be presented by specialised antigen-presenting cells (APCs).
Intracellular antigens: Class I
Intracellular antigens are produced mainly by viruses replicating within a host cell, though antigens here can also derive from cytoplasmic bacteria or the host cell's own proteins. The host cell digests cytoplasmic proteins by a specialized enzyme complex, the proteasome, into small peptides. A specialized carrier, the Transporter associated with Antigen Processing (TAP) complex moves the peptide into the endoplasmic reticulum, allowing the antigenic peptide to be coupled to an MHC Class I molecule and transported to the cell surface.
MHC Class I molecules present antigen to CD8+ cytotoxic T cells and are expressed on all nucleated cells. Cytotoxic T cells (also known as TC, killer T cell, or cytotoxic T-lymphocyte (CTL)) are a population of T cells that are specialized for inducing the death of other cells. Recognition of antigenic peptides through Class I by CTLs leads to the killing of the target cell, which is infected by virus, intracytoplasmic bacterium, or are otherwise damaged or dysfunctional.
Extracellular antigens: Class II
Dendritic cells (DCs) phagocytose exogenous pathogens such as bacteria, parasites, and toxins in the tissues and then migrate, via chemotactic signals, to T cell-enriched lymph nodes. During migration, DCs undergo a process of maturation in which they lose phagocytic capacity and develop an increased ability to communicate with T-cells in the lymph nodes. This maturation process is dependent on signaling from other pathogen-associated molecular pattern (PAMP) molecules through pattern recognition receptors, such as the members of the Toll-like receptor family.
The DC uses lysosome-associated enzymes to digest pathogen-associated proteins into smaller peptides. In the lymph node, the DC will display these antigenic peptides on its surface by coupling them to MHC Class II molecules. This MHC:antigen complex is then recognized by T cells passing through the lymph node. Exogenous antigens are usually displayed on MHC Class II molecules, which interact with CD4+ helper T cells. CD4+ lymphocytes, or TH, are immune response mediators, and play an important role in establishing and maximizing the capabilities of the adaptive immune response.
Expression of Class II is more restricted than Class I. High levels of Class II are found on dendritic cells, but can also be observed on activated macrophages, B cells, and several other host cell types in inflammatory conditions.
- ImmPort - Gene summaries, ontologies, pathways, protein/protein interactions and more for genes involved in antigen processing and presentation
- antigen presentation at the US National Library of Medicine Medical Subject Headings (MeSH)