Influenced by famous thinkers such as Leibniz and Conti he belonged to the Galilean school of experimental scientists. He worked in biology, botany, veterinary medicine, hydrology and the newly born science geology.
Vallisneri died in Padua in 1730.
He is known for being one of the first researchers in medicine to have proposed abandoning the Aristotelian theories for an experimental approach based on the scientific principles suggested by Galileo Galilei. Vallisneri stated that scientific knowledge is best acquired through experience and reasoning. This principle was followed in his anatomical dissections and carefully drawn descriptions of insects. For this reason, his medical career was at the center of heated controversy, as many of his contemporaries could not abandon prevailing medieval theories, even in the face of glaring experimental evidence.
He also was keenly interested in the natural sciences, and over his lifetime collected numerous specimens of animals, minerals and other natural objects. Unfortunately his scientific method was limited when it came to interpreting fossil evidence on mountain tops; the only possibility he allowed for was a miraculous Biblical Flood (Flood geology) as the cause for their deposition.
He had a very clear and precise style of writing. In 1709 he joined Francesco Scipio Maffei and Apostolo Zeno in editing a literary journal, Giornale de' Letterati d'Italia, which had a brief life. Vallisneri's contribution to the use of language makes him one of the most admired science writers, in the tradition of Galilei, Francesco Redi and Lorenzo Magalotti. Vallisneri also followed Galilei's tracks in electing Italian as the language of choice for writing his treatises. This was a courageous choice in the scientific community of the time, which still used Latin as the “language of knowledge.”
- Antonio Vallisnieri (1721), Istoria della generazione dell'uomo, e degli animali, se sia da'vermicelli spermatici, o dalle uova, Venice: Appresso Giovanni Gabbriel Hertz
- Antonio Vallisnieri (1726), Lezione accademica intorno l'origine delle fontane, Venice: Appresso Pietro Poletti, all'Eloquenza in Merceria di S. Salvatore, OCLC 23450508
- Antonio Vallisnieri (1728), Dr corpi marini che su' Monti si trovano; della loro origine, e dello stato del mondo avanti il Diluvio, nel Diluvio, e dopo il Diluvio: Lettere Critiche. (2nd edition, first published 1721, On Marine (fossil) Life to be Found on Mountains ed.), Venice: Domenico Lovisa
|Perennial Springs||1715||On the origin of rivers, based on an exploration in the Tuscan Emilian Apennines|
|Dialoghi sopra la curiosa origine di molti Insetti||"Dialogues on the curious origin of several insects"|
- Curiosity and Ingenuity Link to a virtual exhibition of the collections of Vallisneri, held by the Museums of the University of Padova.