Agnihotri was born on 2 December 1972 in India.He did his schooling from sheiling house School in Kanpur.He received his Bachelor of Arts degree from Bhavan's College, Andheri, Mumbai in 1993. He married Shilpa Sakhlani in 2005.
Apurva Agnihotri debuted in the film Pardes, co-starring with Shahrukh Khan and directed by Subhash Ghai. The film was highly successful and garnered Agnihotri with the nomination for Best Supporting Actor in 1997's Zee Cine Awards. Apurva along with his wife are participants of reality show Big Boss 7 which has been aired on Colors from 15th Sept 2013. In the starting episode, Apurva has been sent to Jahannum('Hell') while his wife was assigned Jannat ('Heaven').
He joined the show Bigg Boss 7 on 15 September 2013 with his wife Shilpa Sakhlani. He was a patient and calm player and also shaved his hair off to make his team win a task. He was voted out by the audience on the 7th week of the show (2 November 2013)