Auramine O
| Auramine O | |
|---|---|
 |
|
 Solid Auramine O
|
 Auramine O in aqueous solution
|
|
bis[4-(dimethylamino)phenyl]methaniminium chloride |
|
|
Other names
Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000 |
|
| Identifiers | |
| CAS number | 2465-27-2  |
| PubChem | 17170 |
| ChemSpider | 16254 |
| Jmol-3D images | Image 1 Image 2 |
|
|
|
|
| Properties | |
| Molecular formula | C17H22ClN3 |
| Molar mass | 303.83 g mol−1 |
| Melting point |
267 °C |
| Hazards | |
| R-phrases | R22 R24 R40 |
| S-phrases | S36/37 S45 |
 (verify) (what is:  / ?)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
|
| Infobox references | |
Auramine O is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals. It is very soluble in water and soluble in ethanol.
Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium, where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain.[1] It can also be used as a fluorescent version of Schiff reagent.[2]
Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis.[3][4] It can be also used as an antiseptic agent. According to the packaging of Post-it brand notes, Canary Yellow is a trademark of 3m.
References [edit]
- ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085–9. doi:10.1016/S0046-8177(84)80253-1. PMID 6208117.
- ^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B (1980). "Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli". Gastroenterology 78 (4): 782–90. PMID 6986320.
- ^ Truant J, Brett W, Thomas W (1962). "Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine". Henry Ford Hosp Med Bull 10: 287–96. PMID 13922644.
- ^ Arrowood M, Sterling C (1989). "Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection". J Clin Microbiol 27 (7): 1490–5. PMC 267601. PMID 2475523.
External links [edit]
|
||||||||||||||||||||||||||
