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The rabbit test, or Aschheim-Zondek test, was an early pregnancy test developed in 1927 by Bernhard Zondek and Selmar Aschheim. The original test actually used mice, and was based upon the observation that when urine from a female in the early months of pregnancy is injected into immature female mice, the ovaries of the mice enlarge and show follicular maturation. The test was considered reliable, with an error rate of less than 2%. The rabbit test consisted of injecting the tested woman's urine into a female rabbit, then examining the rabbit's ovaries a few days later, which would change in response to a hormone only secreted by pregnant women. The hormone, human chorionic gonadotropin (hCG), is produced during pregnancy and indicates the presence of a fertilized egg; it can be found in a pregnant woman's urine and blood. The rabbit test became a widely used bioassay (animal-based test) to test for pregnancy. The term "rabbit test" was first recorded in 1949 but became a common phrase in the English language. Xenopus frogs were also used in a similar "frog test".
Modern pregnancy tests still operate on the basis of testing for the presence of the hormone hCG. Due to medical advances, use of a live animal is no longer required.
It is a common misconception that the injected rabbit would die only if the woman was pregnant. This led to the phrase "the rabbit died" being used as a euphemism for a positive pregnancy test. In fact, all rabbits used for the test died, because they had to be surgically opened in order to examine the ovaries. While it was possible to do this without killing the rabbit, it was generally deemed not worth the trouble and expense.
In popular culture 
- An independent short film titled Redemption Maddie centers on the idea of testing pregnancy with rabbits.
- Billy Crystal starred in a 1978 movie Rabbit Test in which his character became pregnant.
- In the sixth episode of the second season of The Dick Van Dyke Show, Mary Tyler Moore's character Laura says "the rabbit died" to convey the news to Rob that she is pregnant.
- A collection of essays by Teresa Bloomingdale, columnist and mother of ten, bears the title I Should Have Seen It Coming When the Rabbit Died (1979). The title refers to an anecdote (1956) in which the rabbit used in her first pregnancy test died from jumping off the examination table, thereby shrugging off any responsibility for her ever-growing family.[clarification needed]
- In the M*A*S*H episode, "What's Up, Doc" (S6, Ep19), Hawkeye removed the ovaries from Radar's rabbit "Fluffy" to run the test when Maj. Margaret Houlihan suspected she was pregnant.
- In the Showtime dramedy Californication, two of the main characters (Hank and Marcy) each use the phrase "the rabbit done died" in separate episodes when they are not pleased with a positive pregnancy test.
- In the Squidbillies episode "Family Trouble",first aired November 13, 2005, the rabbit test is referenced in a paternity suit.
- In the television show House (Season 5, Episode 8), the character House mentions the rabbit test saying "[since a] pregnancy test only takes 5 minutes and we no longer kill rabbits [...] do the test."
- In the song "Sweet Emotion" by Aerosmith the lyrics include the line "You can't catch me cause the rabbit done died."
- In the 1996 film Grace of My Heart starring Illeana Douglas, her character tells her boyfriend she "is just waiting to see if the rabbit dies" while discussing her possible pregnancy.
- In the 1987 film Fatal Attraction, the character Alex Forrest (Glenn Close) boils the pet rabbit belonging to the daughter of the man with whom she is having an affair (played by Michael Douglas) to communicate symbolically that she is pregnant.
- Morris Fishbein, M.D., ed. (1976). "Aschheim-Zondek Test". The New Illustrated Medical and Health Encyclopedia 1 (Home Library Edition ed.). New York, N.Y. 10016: H. S. Stuttman Co. p. 139.
- Evans, Herbert M.; Simpson, Miriam E. (1930). "Aschheim-Zondek test for pregnancy - its present status.". Calif West Med 32 (3): 145–8. PMC 1657362. Retrieved 2009-02-09.