|Micrograph of A. niger grown on Sabouraud agar medium. 100x magnification.|
van Tieghem 1867
Aspergillus niger var. niger
Aspergillus niger or A. niger is a fungus and one of the most common species of the genus Aspergillus. It causes a disease called black mold on certain fruits and vegetables such as grapes, onions, and peanuts, and is a common contaminant of food. It is ubiquitous in soil and is commonly reported from indoor environments, where its black colonies can be confused with those of Stachybotrys (species of which have also been called "black mould").
Some strains of A. niger have been reported to produce potent mycotoxins called ochratoxins, but other sources disagree, claiming this report is based upon misidentification of the fungal species. Recent evidence suggests some true A. niger strains do produce ochratoxin A. It also produces the isoflavone orobol.
A. niger is included in Aspergillus subgenus Circumdati, section Nigri. The section Nigri includes 15 related black-spored species that may be confused with A. niger, including A. tubingensis, A. foetidus, A. carbonarius, and A. awamori. A number of morphologically similar species were recently described by Samson et al.
Recently the strain of ATCC 16404 Aspergillus niger has been reclassified at Aspergillus brasiliensis (refer to publication by Varga et al.). This has required an update to the U.S. Pharmacopoeia and the European Pharmacopoeia which commonly use this strain throughout the pharmaceutical industry.
Plant disease 
A. niger causes black mold of onions. Infection of onion seedlings by A. niger can become systemic, manifesting only when conditions are conducive. A. niger causes a common postharvest disease of onions, in which the black conidia can be observed between the scales of the bulb. The fungus also causes disease in peanuts and in grapes.
Human and animal disease 
A. niger is less likely to cause human disease than some other Aspergillus species, but, if large amounts of spores are inhaled, A. niger can be deadly. This is due to a serious lung disease, aspergillosis, that can occur. Aspergillosis is, in particular, frequent among horticultural workers that inhale peat dust, which can be rich in Aspergillus spores. It has been found in the mummies of ancient Egyptian tombs and can be inhaled when they are disturbed.
Industrial uses 
A. niger is cultured for the industrial production of many substances. Various strains of A. niger are used in the industrial preparation of citric acid (E330) and gluconic acid (E574) and have been assessed as acceptable for daily intake by the World Health Organisation. A. niger fermentation is "generally recognized as safe" (GRAS) by the United States Food and Drug Administration under the Federal Food, Drug, and Cosmetic Act.
Many useful enzymes are produced using industrial fermentation of A. niger. For example, A. niger glucoamylase is used in the production of high fructose corn syrup, and pectinases are used in cider and wine clarification. Alpha-galactosidase, an enzyme that breaks down certain complex sugars, is a component of Beano and other products that decrease flatulence. Another use for A. niger within the biotechnology industry is in the production of magnetic isotope-containing variants of biological macromolecules for NMR analysis.
Other uses 
A. niger is also cultured for the extraction of the enzymes glucose oxidase (GO) and Alpha-galactosidase (AGS). Glucose oxidase is used in the design of glucose biosensors, due to its high affinity for β-D-glucose. Alpha-galactosidase can be produced by A. niger fermentation; it is used to hydrolyze alpha 1-6 bonds found in melibiose, raffinose, and stachyose.
Research published in 2006-2008 investigated A. niger prolyl endoprotease (AN-PEP), a microbial-derived prolyl endoprotease which cleaves gluten. This has strong implications in the treatment of coeliac disease or other metabolic gluten sensitivity disease processes. A placebo controlled, double blind study was initiated in December 2008 to determine the efficacy of this enzyme in treating humans with coeliac disease.
A. niger is often used as a challenge organism for cleaning validation studies performed within sterile manufacturing facilities.
See also 
- Samson RA, Houbraken J, Summerbell RC, Flannigan B, Miller JD (2001). Common and important species of fungi and actinomycetes in indoor environments. In: Microogranisms in Home and Indoor Work Environments. New York: Taylor & Francis. pp. 287–292. ISBN.
- Abarca M, Bragulat M, Castellá G, Cabañes F (1994). "Ochratoxin A production by strains of Aspergillus niger var. niger". Appl Environ Microbiol 60 (7): 2650–2. PMC 201698. PMID 8074536.
- Schuster E, Dunn-Coleman N, Frisvad JC, Van Dijck PW (August 2002). "On the safety of Aspergillus niger--a review". Applied microbiology and biotechnology 59 (4–5): 426–35. doi:10.1007/s00253-002-1032-6. PMID 12172605.
- Klich MA (2002). Identification of common Aspergillus species. Utrecht, The Netherlands, Centraalbureau voor Schimmelcultures. ISBN 90-70351-46-3.
- Samson, RA, Houbraken JAMP, Kuijpers AFA, Frank JM, Frisvad JC (2004). "New ochratoxin A or sclerotium producing species in Aspergillus section Nigri". Studies in Mycology 50: 45–6.
- Varga et al. (2007). International Journal of Systematic and Evolutionary Microbiology 57: 1925–1932.
- Handwerk, Brian (May 6, 2005) Egypt's "King Tut Curse" Caused by Tomb Toxins? National Geographic News and National Geographic Channel
- "Inventory of GRAS Notices: Summary of all GRAS Notices". US FDA/CFSAN. 2008-10-22. Archived from the original on 11 October 2008. Retrieved 2008-10-31.
- Petro, Mike. "Pu-erh, A Westerner's Quest". Retrieved 2008-07-10
- Staiano, Maria, Paolo Bazzicalupo, Mose' Rossi, and Sabato D'Auria. “Glucose biosensors as models for the development of advanced protein-based biosensors.” Molecular BioSystems 1 (2005): 354-362.
- Mitea C, Havenaar R, Drijfhout JW, Edens L, Dekking L, Koning F (2008). "Efficient degradation of gluten by a prolyl endoprotease in a gastrointestinal model: implications for coeliac disease". Gut. Jan;57 (1): 25–32. doi:10.1136/gut.2006.111609. PMID 17494108.
- Mulder, C.J. (December). "Effect of Aspergillus Niger Prolyl Endoprotease (AN-PEP) Enzyme on the Effects of Gluten Ingestion in Patients With Coeliac Disease". Archived from the original on 4 September 2009. Retrieved 2009-10-07
- Calmette, L.C.A. (1892). "La fermentation de l'opium des fumeurs". Arch. Méd. Navale coloniale 57: 132–138.
- Challenege Organisms. "Appendix 2: Challenge test of water miscible cosmetic products".
- Pel H, de Winde J, Archer D, et al. (2007). "Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88". Nat Biotechnol 25 (2): 221–31. doi:10.1038/nbt1282. PMID 17259976.
- Andersen MR, Salazar MP, Schaap PJ, et al. (2011). "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88". Genome Res 21 (6): 885–97. doi:10.1101/gr.112169.110. PMC 3106321. PMID 21543515.
- A. Niger ATCC 1015 genome
- Aspergillus website (Manchester University, UK)
- Aspergillosis information (Center for Disease Control)
- World Health Organisation 1975 report on microbial carbohydrase enzyme prepared from Aspergillus niger, as a food additive