Born in Woodbrook, Port of Spain to an upper-middle-class family, Jeffers was educated at Tranquillity Girls School and went to England when she was aged 15, later taking a diploma in social science at Alexander College, north London. While in London she was involved in founding the Union of Students of African Descent, which would become known as the League of Coloured Peoples. After the outbreak of the First World War she worked among West African troops and set up a West African soldiers' fund, mobilising financial contributions from fellow West Indians.
She returned to Trinidad in 1920 and ran a junior school in her family home, Briarsend. Moved by the sufferings of the underprivileged and dispossessed, she established the Coterie of Social Workers, which provided free lunches to poor school children. The first "Breakfast Shed" was established in Port of Spain in 1926. Others were established in Barataria, San Fernando, Trinidad and Tobago, Siparia and Tobago. They went on to establish homes for the elderly, the blind, "women in distress" and day nurseries. The first day nursery, established in John John, Port of Spain, was named Cipriani House after the labour leader Arthur Andrew Cipriani.
The Audrey Jeffers Highway is named after her.
- Anthony, Michael (2001). Historical Dictionary of Trinidad and Tobago. Scarecrow Press, Inc. Lanham, Md., and London. ISBN 0-8108-3173-2.
- Comma-Maynard, Olga, The Briarend Pattern: The Story of Audrey Jeffers O.B.E. and the Coterie of Social Workers, Port of Spain: Busby's Printery, 1971.
- Wieringa, Saskia (ed.), Subversive Women: Women's Movements in Africa, Asia, Latin America and the Caribbean, Chapter 5, London: Zed Books, 1995.
- "Audrey Jeffers (1896-1968), Profiles - Heroes, Pioneers and Role Models of Trinidad and Tobago, Safari Publications, pp. 47-8.
- Helen Rappaport, Encyclopedia of Women Social Reformers, Vol. 1, A - L. Santa Barbara, California: ABC-CLIO, 2001, p. 335.
- "Martin, Professor Anthony", Death Notices, Trinidad & Tobago Guardian.
- Jeffrey Green, "Slow March – Left, Right", BASA (Black & Asian Studies Association) Newsletter, Diamond # 60 Issue - July 2011 # 61 November 2011.