Auramine-rhodamine stain

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Oocysts of Cryptosporidium parvum stained with the fluorescent auramine-rhodamine stain.

The auramine-rhodamine stain (AR), also known as the Truant auramine-rhodamine stain, is a histological technique used to visualize acid-fast bacilli using fluorescence microscopy, notably species in the Mycobacterium genus.[1] Acid-fast organisms display a reddish-yellow fluorescence.[2] Although the auramine-rhodamine stain is not as specific for acid-fast organisms (i.e. Mycobacterium tuberculosis or Nocardia) as the Ziehl-Neelsen stain, it is more affordable and more sensitive, therefore it is often utilized as a screening tool.

AR stain is a mixture of Auramine O and Rhodamine B.

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References[edit]

  1. ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085–9. doi:10.1016/S0046-8177(84)80253-1. PMID 6208117. 
  2. ^ Abe, C. (August 2003). "結核とそれらの上達テストの実験室テストの標準化" [Standardization of laboratory tests for tuberculosis and their proficiency testing]. Kekkaku (in Japanese) 78 (8): 541–551. ISSN 0022-9776. PMID 14509226.