She danced nearly all of the principal roles in ballets by Didelot. She became the first Russian dancer en pointe. She served in the Imperial Ballet for twenty years. She married a young actor, but he soon died. Late in life she married once again, this time to the dramatic actor Pavel Ekunin (ru: Павел Семенович Экунин). By this time she was no longer performing leading roles and her wage was reduced by half. In the 1835/1836 season, she was injured during the performance and was asked to leave for treatment. Emperor Nicholas I of Russia saw this request and wrote the order: to be dismissed. On January 30, 1836 Avdotya Istomina came on stage one last time and danced a little Russian dance.
She died of cholera on June 26 (July 8) in 1848 in St. Petersburg. No one could remember her as a famous ballerina. The inscription on her tomb: "Avdotya Ilyinichna Ekunina, a retired actress".