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B16 or B-16 may refer to:
- B16 (New York City bus) serving Brooklyn
- HLA-B16, an HLA - B serotype
- Honda B16 engine
- Volvo B16 engine
- Martin XB-16, a proposed bomber aircraft
- B16 Mouse melanoma cell line
- One of the ECO codes for the Caro–Kann Defence in chess
- An informal reference to Pope Benedict XVI
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