B3GAT2

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Beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)
Protein B3GAT2 PDB 2d0j.png
PDB rendering based on 2d0j.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols B3GAT2 ; GLCATS
External IDs OMIM607497 MGI2389490 HomoloGene50574 ChEMBL: 5209 GeneCards: B3GAT2 Gene
EC number 2.4.1.135
Orthologs
Species Human Mouse
Entrez 135152 280645
Ensembl ENSG00000112309 ENSMUSG00000026156
UniProt Q9NPZ5 P59270
RefSeq (mRNA) NM_080742 NM_172124
RefSeq (protein) NP_542780 NP_742122
Location (UCSC) Chr 6:
71.57 – 71.67 Mb
Chr 1:
23.76 – 23.85 Mb
PubMed search [1] [2]

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 is an enzyme that in humans is encoded by the B3GAT2 gene.[1][2]

The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family, and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1) carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in the nervous system.[2]

Use of HNK-1 Antibody for Neural Crest Research[edit]

Antibodies raised against the HNK-1 epitope have played a large role in studies of the neural crest, especially in the avian embryo.[3] The first antibody raised against this epitope was NC-1,[4] which permitted much easier analyses of neural crest migration pathways. In avians, and especially in other vertebrates, the results of HNK-1 staining should be interpreted with caution as the epitope is not unique to the neural crest.

References[edit]

  1. ^ Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, Kawasaki T (Oct 2002). "cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope". Gene 296 (1-2): 29–36. doi:10.1016/S0378-1119(02)00840-5. PMID 12383500. 
  2. ^ a b "Entrez Gene: B3GAT2 beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)". 
  3. ^ Bronner-Fraser M (May 1986). "Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1". Dev. Biol. 115 (1): 44–55. doi:10.1016/0012-1606(86)90226-5. PMID 3516760. 
  4. ^ Vincent M, Thiery JP (June 1984). "A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system". Dev. Biol. 103 (2): 468–81. doi:10.1016/0012-1606(84)90334-8. PMID 6202575. 

Further reading[edit]