5-Bromo-4-chloro-3-indolyl phosphate
| BCIP | |
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(5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate |
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Other names
BCIP |
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| Identifiers | |
| CAS number | 38404-93-2  |
| PubChem | 65409 |
| Jmol-3D images | Image 1 |
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| Properties | |
| Molecular formula | C8H6BrClNO4P |
| Molar mass | 326.47 g mol−1 |
| Appearance | Colorless |
| Solubility in water | soluble in water (sodium salt) |
 (verify) (what is:  / ?)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
5-Bromo-4-chloro-3-indolyl phosphate (BCIP, X-phosphate, XP) is an artificial chromgenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It is for example used in immunoblotting, in situ hybridization, and immunohistochemistry, often in combination with nitro blue tetrazolium chloride (NBT).[1] Alkaline phosphatase hydrolyses BCIP to 5-bromo-4-chloro-3-indole and inorganic phosphate. 5-bromo-4-chloro-3-indole is oxidized by atmospheric oxygen to form the blue dye 5,5′-dibromo-4,4′-dichloro-indigo. It is also oxidized by nitroblue tetrazolium (NBT), which forms an insoluble dark blue diformazan precipitate after reduction. Alkaline phosphatase is commonly conjugated to secondary antibodies.
