BUB1B

Budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Identifiers
Symbols	BUB1B; BUB1beta; BUBR1; Bub1A; MAD3L; SSK1; hBUBR1
External IDs	OMIM: 602860 MGI: 1333889 HomoloGene: 933 GeneCards: BUB1B Gene
EC number	2.7.11.1
Gene Ontology Molecular function • nucleotide binding • protein kinase activity • protein serine/threonine kinase activity • protein binding • ATP binding Cellular component • kinetochore • kinetochore • kinetochore • kinetochore • condensed chromosome kinetochore • condensed chromosome outer kinetochore • nucleus • anaphase-promoting complex • chromosome • cytoplasm • microtubule organizing center • cytosol • cytosol • cytoskeleton • perinuclear region of cytoplasm • spindle midzone Biological process • cell cycle checkpoint • M phase of mitotic cell cycle • mitotic prometaphase • mitotic cell cycle • protein phosphorylation • protein phosphorylation • apoptosis • cell cycle • spindle organization • mitosis • mitotic metaphase/anaphase transition • mitotic cell cycle checkpoint • mitotic cell cycle spindle assembly checkpoint • cell proliferation • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • protein localization to kinetochore • phosphatidylinositol-mediated signaling • cell division • negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	701	12236
Ensembl	ENSG00000156970	ENSMUSG00000040084
UniProt	O60566	Q3UZD6
RefSeq (mRNA)	NM_001211.5	NM_009773.3
RefSeq (protein)	NP_001202.4	NP_033903.2
Location (UCSC)	Chr 15: 40.45 – 40.51 Mb	Chr 2: 118.42 – 118.47 Mb
PubMed search	[1]	[2]

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene.

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Interactions

BUB1B has been shown to interact with AP2B1, HDAC1, BUB3, MAD2L1, Gamma-synuclein, BRCA2 and CDC20.

Further reading

• Posas F, Saito H (1998). "Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator". EMBO J. 17 (5): 1385–94. doi:10.1093/emboj/17.5.1385. PMC 1170486. PMID 9482735. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170486. 
• Cahill DP, Lengauer C, Yu J, et al. (1998). "Mutations of mitotic checkpoint genes in human cancers". Nature 392 (6673): 300–3. doi:10.1038/32688. PMID 9521327. 
• Donadelli R, Benatti L, Remuzzi A, et al. (1998). "Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress". Biochem. Biophys. Res. Commun. 246 (3): 881–7. doi:10.1006/bbrc.1998.8713. PMID 9618306. 
• Taylor SS, Ha E, McKeon F (1998). "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase". J. Cell Biol. 142 (1): 1–11. doi:10.1083/jcb.142.1.1. PMC 2133037. PMID 9660858. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2133037. 
• Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132809. 
• Jablonski SA, Chan GK, Cooke CA, et al. (1999). "The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis". Chromosoma 107 (6–7): 386–96. doi:10.1007/s004120050322. PMID 9914370. 
• Cahill DP, da Costa LT, Carson-Walter EB, et al. (1999). "Characterization of MAD2B and other mitotic spindle checkpoint genes". Genomics 58 (2): 181–7. doi:10.1006/geno.1999.5831. PMID 10366450. 
• Chan GK, Jablonski SA, Sudakin V, et al. (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2169490. 
• Li W, Lan Z, Wu H, et al. (2000). "BUBR1 phosphorylation is regulated during mitotic checkpoint activation". Cell Growth Differ. 10 (11): 769–75. PMID 10593653. 
• Futamura M, Arakawa H, Matsuda K, et al. (2000). "Potential role of BRCA2 in a mitotic checkpoint after phosphorylation by hBUBR1". Cancer Res. 60 (6): 1531–5. PMID 10749118. 
• Wu H, Lan Z, Li W, et al. (2000). "p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase". Oncogene 19 (40): 4557–62. doi:10.1038/sj.onc.1203803. PMID 11030144. 
• Saffery R, Irvine DV, Griffiths B, et al. (2001). "Components of the human spindle checkpoint control mechanism localize specifically to the active centromere on dicentric chromosomes". Hum. Genet. 107 (4): 376–84. doi:10.1007/s004390000386. PMID 11129339. 
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083732. 
• Skoufias DA, Andreassen PR, Lacroix FB, et al. (2001). "Mammalian mad2 and bub1/bubR1 recognize distinct spindle-attachment and kinetochore-tension checkpoints". Proc. Natl. Acad. Sci. U.S.A. 98 (8): 4492–7. doi:10.1073/pnas.081076898. PMC 31862. PMID 11274370. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=31862. 
• Kaplan KB, Burds AA, Swedlow JR, et al. (2001). "A role for the Adenomatous Polyposis Coli protein in chromosome segregation". Nat. Cell Biol. 3 (4): 429–32. doi:10.1038/35070123. PMID 11283619. 
• Sudakin V, Chan GK, Yen TJ (2001). "Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2". J. Cell Biol. 154 (5): 925–36. doi:10.1083/jcb.200102093. PMC 2196190. PMID 11535616. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2196190. 
• Tang Z, Bharadwaj R, Li B, Yu H (2001). "Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1". Dev. Cell 1 (2): 227–37. doi:10.1016/S1534-5807(01)00019-3. PMID 11702782. 
• Shichiri M, Yoshinaga K, Hisatomi H, et al. (2002). "Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival". Cancer Res. 62 (1): 13–7. PMID 11782350. 
• Taylor SS, Hussein D, Wang Y, et al. (2002). "Kinetochore localisation and phosphorylation of the mitotic checkpoint components Bub1 and BubR1 are differentially regulated by spindle events in human cells". J. Cell. Sci. 114 (Pt 24): 4385–95. PMID 11792804.