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Banana spider may refer to:
- Golden silk orb-weaver, a widespread homogenus of large but rather harmless spiders, noted for their large durable webs
- Argiope appensa, a black and yellow spider on several islands in the Western Pacific Ocean
- Brazilian wandering spider, a South and Central American genus of extremely venomous spiders
- Banana spider myth, an urban legend regarding Huntsman spiders
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