Barlow Trail is a major arterial road in Calgary, Alberta, Canada. It is composed of multiple parts: Barlow Trail North extends north to the city limits (though Barlow Trail is a rural road north of Country Hills Boulevard), where Deerfoot Trail takes its alignment out of the city, and south to 17 Avenue SE, while Barlow Trail S leads from Deerfoot Trail at the intersection with Peigan Trail to Deerfoot Trail near the intersection with 130 Avenue SE. The portions are connected by 26 Street SE to form one continuous route.
Barlow Trail North used to be the primary road to Calgary International Airport. On April 3, 2011, the section of Barlow Trail between Airport Trail NE and 48th Avenue NE was permanently closed in order to provide space for a fourth runway at the airport; the primary north-south entrance to the airport was diverted to Airport Trail via Deerfoot Trail to the west.