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A Basophil granulocyte stains dark purple upon H&E staining.

Basophilic is a technical term used by histologists. It describes the microscopic appearance of cells and tissues, as seen down the microscope, after a histological section has been stained with a dye attracted to basic molecules. The most common such dye is haematoxylin.

Basophilic describes the appearance of structures seen in histological sections which take up basic dyes. The structures usually stained are those that contain negative charges, such as the phosphate backbone of DNA in the cell nucleus and ribosomes.

Basophils are cells that "love" the blue, and usually show up deep blue under standard staining techniques (H&E). Specifically, this term refers to:

Different definitions will apply to basophilic:

Basophilic stains are acidic stains called base loving stains. Basophilic cells are base loving cells. Basophilic cells attract base dyes, such as haematoxylin; however, 'Eosin' is referred to as an basophilic stain.

Simplistically, acid pH stains (positive charge) are attracted to the base pH tissue(negative charge), so they are called basophilic stains. Eosinophils (base components that like acids) are dyed red by the basophilic stain or acid stain, eosin. Basophils(acid that like base components) are dyed blue by the acidophilic or basic stain, hematoxylin.

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