Beta-galactoside alpha-2,6-sialyltransferase

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beta-galactoside alpha-2,6-sialyltransferase
Identifiers
EC number 2.4.99.1
CAS number 9075-81-4
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, a beta-galactoside alpha-2,6-sialyltransferase (EC 2.4.99.1) is an enzyme that catalyzes the chemical reaction

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine \rightleftharpoons CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D- glucosamine

Thus, the two substrates of this enzyme are CMP-N-acetylneuraminate and beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine, whereas its 3 products are CMP, alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-, and glucosamine.

This enzyme belongs to the family of transferases, specifically those glycosyltransferases that do not transfer hexosyl or pentosyl groups. The systematic name of this enzyme class is CMP-N-acetylneuraminate:beta-D-galactosyl-1,4-N-acetyl-beta-D-glucos amine alpha-2,6-N-acetylneuraminyltransferase. This enzyme participates in n-glycan biosynthesis and glycan structures - biosynthesis 1.

References[edit]

  • Bartholomew BA, Jourdian GW, Roseman S (1973). "The sialic acids. XV. Transfer of sialic acid to glycoproteins by a sialyltransferase from colostrum". J. Biol. Chem. 248 (16): 5751–62. PMID 4723915. 
  • Hickman J, Ashwell G, Morell AG, van den Hamer CJ, Scheinberg IH (1970). "Physical and chemical studies on ceruloplasmin. 8. Preparation of N-acetylneuraminic acid-1-14C-labeled ceruloplasmin". J. Biol. Chem. 245 (4): 759–66. PMID 4313609. 
  • Paulson JC, Beranek WE, Hill RL (1977). "Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose". J. Biol. Chem. 252 (7): 2356–62. PMID 849932. 
  • Schachter H, Narasimhan S, Gleeson P, Vella G (1983). "Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type". Methods Enzymol. 98: 98–134. doi:10.1016/0076-6879(83)98143-0. PMID 6366476. 
  • Spiro MJ, Spiro RG (1968). "Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase". J. Biol. Chem. 243 (24): 6520–8. PMID 5726897.