||This article may be too technical for most readers to understand. (February 2012)|
|Restriction endonuclease BglII|
structure of restriction endonuclease BstYI bound to non-cognate DNA
Like most type II restriction enzymes, BglII consists of two identical subunits that form a homodimer around the DNA double helix. Each monomer is 223 amino acids and symmetrically bind both sides of the unique palindromic nucleotide sequence AGATCT, cleaving the scissile phosphodiester bond between the first Adenine and Guanine nucleotides on both strands of the DNA molecule, creating sticky ends with 5' end overhangs.
Being a type II restriction enzyme, BglII does not require ATP (adenosine triphosphate) for its enzymatic function, but only requires association with a divalent metal cation, most likely Mg2+. Unlike other restriction enzymes of its class, BglII has been show to possess some unique structural characteristics, such as a β-sandwich subdomain, and appears to undergo a unique conformational change upon dimerization, but its overall structure and mechanism of catalysis remain consistent with other type II restriction enzymes.
Restriction Endonuclease enzymes play a very important role in modern molecular cloning techniques. Because of their unique recognition/cut sites, restriction enzymes can be used to precisely and controlably cut DNA in specific locations. Once cut, scientist can then insert the desired DNA fragment possessing "sticky ends" into a circular DNA molecule, and ligate them together to create an engineered cloning vector.
|Name||BglII Restriction Endonuclease|
|This phosphoryl transfer occurs by a nucleophilic attack of a hydride ion on the scissile phosphate, resulting in a trigonal bipyramidal phosphorus intermediate. The phosphorus then gets substituted and the 3'-0- is kicked off as a leaving group.|
BglII catalyses phosphodiester bond cleavage at the DNA backbone through a phosphoryl transfer to water. Studies on the mechanism of restriction enzymes have revealed several general features that seem to be true in almost all cases, although the actual mechanism for each enzyme is most likely some variation of this general mechanism. This mechanism requires a base to generate the hydroxide ion from water, which will act as the nucleophile and attack the phosphorus in the phosphodiester bond. Also required is a Lewis acid to stabilize the extra negative charge of the pentacoordinated transition state phosphorus, as well as a general acid or metal ion that stabilizes the leaving group (3’-O-).
Although restriction endonucleases show little sequence similarity, crystal structures reveal that they all share a highly similar α/β core consisting of a six-stranded β-sheet flanked by five α-helicies, two of which mediate dimerization. This core carries the active site (catalytic center) and the residues that contact DNA in the major groove. BglII is unique in that its α/β core is augmented by a β-sandwich subdomain that has several projections that extend outward to grip the DNA, allowing BglII to completely encircle the DNA molecule. This atypical feature of BglII suggests a unique hinge motion for DNA binding and release. Comparative structural studies of the free enzyme vs. the BglII-DNA complex showed that the enzyme opens by a dramatic scissor-like motion, accompanied by a complete rearrangement of the α-helicies at the dimer interface. These structural studies also revealed that within each monomer a set of residues lowers or raises to alternatively sequester or expose the active site residues. These dramatic differences in structure in the free vs. bound enzyme have yet to be observed in any other restriction endonuclease and may possibly represent a novel mechanism for capturing DNA that may extend to other proteins that encircle DNA.
Structural studies of endonucleases have revealed a similar architecture for the [active site] with the residues following the weak consensus sequence (Glu/Asp)-X(9-20)-(Glu/Asp/Ser)-X-(Lys/Glu). BglII active site is similar to other endonucleases, but follows the sequence Asp 84-X9-Glu 93-X-Gln 95. In its active site there sits a divalent metal ion, most likely Mg2+, that allows interactions with Asp 84, Val 94, a phosphorus oxygen, and three water molecules. One of these water molecules, which we have labeled Nu, is well equipped to be the source of the hydroxide nucleophile because of its proximity to the scissile phosphate as well as a pKa lowered by its contact with the metal and its orientation fixed by a hydrogen bond with the side chain Oxygen of Gln 95.
- BamHI, a nuclease enzyme from 'Bacillus amyloliquefaciens..
- FokI, a nuclease enzyme from Flavobacterium okeanokoites
- EcoRI, a nuclease enzyme from 'E. coli.
- Lukacs et al.; Kucera, R; Schildkraut, I; Aggarwal, AK (2000). "Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1,5 Å resolution.". Nature Structural & Molecular Biology 7 (2): 134–40. doi:10.1038/72405. PMID 10655616.
- Lukacs et al.; Kucera, R; Schildkraut, I; Aggarwal, AK (2001). "Structure of free BglII reveals an unprecedented scissor-like motion for opening and endonuclease.". Nature Structural & Molecular Biology 8 (2): 126–130. doi:10.1038/84111. PMID 11175900.
- Galburt, E. A., and Stoddard, B. L. (2000). "Restriction endonucleases: one of these things is not like the others.". Nature Structural & Molecular Biology 7 (2): 89–91. doi:10.1038/72450. PMID 10655603.
- Pingoud, Alfred, and Jeltsch, Albert. (2001). "Structure and function of type II restriction endonucleases". Nature Nucleic Acids Research 29 (18): 3705–3727. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
Molecular graphics images were produced using the UCSF Chimera package from the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIH P41 RR-01081).