The library holds about 130 000 volumes of manuscripts, incunabula, and books. In this number about 3 000 manuscripts written in Latin, Greek, among them Bible of Alcuin from the 9th century, lectionary from the 12th century etc. The library holds documents from the time of the Reformation and Counter-Reformation.
The library was established in 1565 by Filippo Neri. In 1699 Leone Allacci presented to the library 243 volumes, 137 Latin and 106 Greek (Fondo Allacci), in 1764 Giuseppe Bianchini presented 293 volumes (Fondo Bianchini), in 1843 Ruggero Falzacappa presented 79 volumes of documents from 17-19th centuries (Fondo Falzacappa).
- Biblioteca Vallicelliana at the Ministero per i Beni e le Attività Culturali
- The Biblioteca Vallicelliana - Regole Per La Lingua Maltese
- De Leonis Allati Codicibus qui Romae in Biblioteca Vallicelliana asservantur
- List of press marks, Roma, Biblioteca Vallicelliana