Bicinchoninic acid

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Bicinchoninic acid
Structure of bicinchoninic acid.png
Names
IUPAC name
2-(4-Carboxyquinolin-2-yl)quinoline-4-carboxylic acid
Other names
Bicinchoninic acid
4,4'-Dicarboxy-2,2'-biquinoline
Identifiers
1245-13-2 YesY
ChEMBL ChEMBL431482 YesY
ChemSpider 64223 YesY
Jmol-3D images Image
PubChem 71068
Properties
C20H12N2O4
Molar mass 344.32 g·mol−1
Appearance Cream colored powder
Odor Characteristic odor[vague]
Melting point 365 °C (689 °F; 638 K)
Partially soluble in cold water, hot water[vague]
Hazards
MSDS External MSDS
Main hazards Will irritate eyes and mucous membranes.
NFPA 704
Flammability (red): no hazard code Health code 1: Exposure would cause irritation but only minor residual injury. E.g., turpentine Reactivity (yellow): no hazard code Special hazards (white): no codeNFPA 704 four-colored diamond
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid (BCA) assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.[1]

References[edit]

  1. ^ Pierce BCA Protein Assay Kit