PDB rendering based on 2ft3.
|RNA expression pattern|
Biglycan is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. In humans, biglycan is encoded by the BGN gene.
Biglycan consists of a protein core containing leucine-rich repeat regions and two glycosaminoglycan (GAG) chains consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS), with DS being more abundant in most connective tissues. The CS/DS chains are attached at amino acids 5 and 10 in human biglycan. The composition of the GAG chains has been reported as varying according to tissue of origin. Non-glycanated forms of biglycan (no GAG chains) increase with age in human articular cartilage.
The name "biglycan" was proposed in an article by Fisher, Termine and Young in an article in the Journal of Biological Chemistry in 1989 because the proteoglycan contained two GAG chains; formerly it was known as proteoglycan-I (PG-I).
The structure of biglycan core protein is highly conserved across species; over 90% homology has been reported for rat, mouse, bovine and human biglycan core proteins.
Biglycan interacts with collagen, both via the core protein and GAG chains. It has been reported that biglycan interacts more strongly with collagen type II than collagen type I. Biglycan has been reported to compete with decorin for the same binding site on collagen.
Similarity to decorin
Biglycan is believed to play a role in the mineralisation of bone. Knock-out mice that have had the gene for biglycan suppressed have an osteoporosis-like phenotype with reduced growth rate and lower bone mass than mice that can express biglycan.
Biglycan core protein binds to the growth factors BMP-4 and influences its bioactivity. It has also been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan binds to TGF-beta 1
- Traupe H, van den Ouweland AM, van Oost BA, Vogel W, Vetter U, Warren ST, Rocchi M, Darlison MG, Ropers HH (June 1992). "Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel". Genomics 13 (2): 481–3. doi:10.1016/0888-7543(92)90279-2. PMID 1612609.
- Roughley PJ, White RJ (September 1989). "Dermatan sulphate proteoglycans of human articular cartilage. The properties of dermatan sulphate proteoglycans I and II". The Biochemical Journal 262 (3): 823–7. PMC 1133347. PMID 2590169.
- Roughley PJ, White RJ, Magny MC, Liu J, Pearce RH, Mort JS (October 1993). "Non-proteoglycan forms of biglycan increase with age in human articular cartilage". The Biochemical Journal 295 (2): 421–6. PMC 1134898. PMID 8240239.
- Fisher LW, Termine JD, Young MF (March 1989). "Deduced protein sequence of bone small proteoglycan I (biglycan) shows homology with proteoglycan II (decorin) and several nonconnective tissue proteins in a variety of species". The Journal of Biological Chemistry 264 (8): 4571–6. PMID 2647739.
- Schönherr E, Witsch-Prehm P, Harrach B, Robenek H, Rauterberg J, Kresse H (February 1995). "Interaction of biglycan with type I collagen". The Journal of Biological Chemistry 270 (6): 2776–83. doi:10.1074/jbc.270.6.2776. PMID 7852349.
- Pogány G, Hernandez DJ, Vogel KG (August 1994). "The in vitro interaction of proteoglycans with type I collagen is modulated by phosphate". Archives of Biochemistry and Biophysics 313 (1): 102–11. doi:10.1006/abbi.1994.1365. PMID 8053669.
- Vynios DH, Papageorgakopoulou N, Sazakli H, Tsiganos CP (September 2001). "The interactions of cartilage proteoglycans with collagens are determined by their structures". Biochimie 83 (9): 899–906. doi:10.1016/S0300-9084(01)01332-3. PMID 11698112.
- Bidanset DJ, Guidry C, Rosenberg LC, Choi HU, Timpl R, Hook M (March 1992). "Binding of the proteoglycan decorin to collagen type VI". The Journal of Biological Chemistry 267 (8): 5250–6. PMID 1544908.
- Cheng F, Heinegård D, Malmström A, Schmidtchen A, Yoshida K, Fransson LA (October 1994). "Patterns of uronosyl epimerization and 4-/6-O-sulphation in chondroitin/dermatan sulphate from decorin and biglycan of various bovine tissues". Glycobiology 4 (5): 685–96. doi:10.1093/glycob/4.5.685. PMID 7881183.
- Xu T, Bianco P, Fisher LW, Longenecker G, Smith E, Goldstein S, Bonadio J, Boskey A, Heegaard AM, Sommer B, Satomura K, Dominguez P, Zhao C, Kulkarni AB, Robey PG, Young MF (September 1998). "Targeted disruption of the biglycan gene leads to an osteoporosis-like phenotype in mice". Nature Genetics 20 (1): 78–82. doi:10.1038/1746. PMID 9731537.
- Moreno M, Muñoz R, Aroca F, Labarca M, Brandan E, Larraín J (April 2005). "Biglycan is a new extracellular component of the Chordin-BMP4 signaling pathway". The EMBO Journal 24 (7): 1397–405. doi:10.1038/sj.emboj.7600615. PMC 1142540. PMID 15775969.
- Chen XD, Fisher LW, Robey PG, Young MF (June 2004). "The small leucine-rich proteoglycan biglycan modulates BMP-4-induced osteoblast differentiation". The FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology 18 (9): 948–58. doi:10.1096/fj.03-0899com. PMID 15173106.
- Bowe MA, Mendis DB, Fallon JR (2000). "The small leucine-rich repeat proteoglycan biglycan binds to alpha-dystroglycan and is upregulated in dystrophic muscle.". J Cell Biol 148 (4): 801–10. doi:10.1083/jcb.148.4.801. PMC 2169361. PMID 10684260.