Bioreactor

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General structure of batch type bioreactor
Autoclavable bench-top laboratory bioreactor used for fermentation and cell cultures

A bioreactor may refer to any manufactured or engineered device or system that supports a biologically active environment.[1] In one case, a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. This process can either be aerobic or anaerobic. These bioreactors are commonly cylindrical, ranging in size from litres to cubic metres, and are often made of stainless steel.

A bioreactor may also refer to a device or system meant to grow cells or tissues in the context of cell culture. These devices are being developed for use in tissue engineering or biochemical engineering.

On the basis of mode of operation, a bioreactor may be classified as batch, fed batch or continuous (e.g. a continuous stirred-tank reactor model). An example of a continuous bioreactor is the chemostat.

Organisms growing in bioreactors may be suspended or immobilized. Immobilization is a general term describing a wide variety of the cell or the particle attachment or entrapment.[2] It can be applied to basically all types of biocatalysts including enzymes, cellular organelles, animal and plant cells.[3]

Large scale immobilized cell bioreactors are:

Bioreactor design[edit]

A closed bioreactor used in cellulosic ethanol research

Bioreactor design is a relatively complex engineering task, which is studied in the discipline of biochemical engineering. Under optimum conditions, the microorganisms or cells are able to perform their desired function with limited production of impurities. The environmental conditions inside the bioreactor, such as temperature, nutrient concentrations, pH, and dissolved gases (especially oxygen for aerobic fermentations) affect the growth and productivity of the organisms. The temperature of the fermentation medium is maintained by a cooling jacket, coils, or both. Particularly exothermic fermentations may require the use of external heat exchangers. Nutrients may be continuously added to the fermenter, as in a fed-batch system, or may be charged into the reactor at the beginning of fermentation. The pH of the medium is measured and adjusted with small amounts of acid or base, depending upon the fermentation. For aerobic (and some anaerobic) fermentations, reactant gases (especially oxygen) must be added to the fermentation. Since oxygen is relatively insoluble in water (the basis of nearly all fermentation media), air (or purified oxygen) must be added continuously. The action of the rising bubbles helps mix the fermentation medium and also "strips" out waste gases, such as carbon dioxide. In practice, bioreactors are often pressurized; this increases the solubility of oxygen in water.


Fouling can harm the overall sterility and efficiency of the bioreactor, especially the heat exchangers. To avoid it, the bioreactor must be easily cleaned. Interior surfaces are typically made of stainless steel for easy cleaning and sanitation.

In an aerobic process, optimal oxygen transfer is sometimes the most difficult task to accomplish. Oxygen is poorly soluble in water—even less in warm fermentation broths—and is relatively scarce in air (20.95%). Oxygen transfer is usually helped by agitation, which is also needed to mix nutrients and to keep the fermentation homogeneous. There are, however, limits to the speed of agitation, due both to high power consumption (which is proportional to the cube of the speed of the electric motor).

Photobioreactor[edit]

A photobioreactor (PBR) is a bioreactor which incorporates some type of light source. Virtually any translucent container could be called a PBR, however the term is more commonly used to define a closed system, as opposed to an open tank or pond. Photobioreactors are used to grow small phototrophic organisms such as cyanobacteria, algae, or moss plants.[4] These organisms use light through photosynthesis as their energy source and do not require sugars or lipids as energy source. Consequently, risk of contamination with other organisms like bacteria or fungi is lower in photobioreactors when compared to bioreactors for heterotroph organisms.

Sewage treatment[edit]

Bioreactors are also designed to treat sewage and wastewater. In the most efficient of these systems, there is a supply of a free-flowing, chemically inert medium which acts as a receptacle for the bacteria that break down the raw sewage. Examples of these bioreactors often have separate, sequential tanks and a mechanical separator or cyclone to speed the separation of water and biosolids. Aerators supply oxygen to the sewage and medium, further accelerating breakdown. Submersible mixers provide agitation in anoxic bioreactors to keep the solids in suspension and thereby ensure that the bacteria and the organic materials "meet". In the process, the liquid's Biochemical Oxygen Demand (BOD) is reduced sufficiently to render the contaminated water fit for reuse. The biosolids can be collected for further processing, or dried and used as fertilizer. An extremely simple version of a sewage bioreactor is a septic tank whereby the sewage is left in situ, with or without additional media to house bacteria. In this instance, the biosludge itself is the primary host (activated sludge) for the bacteria. Septic systems are best suited where there is sufficient landmass, and the system is not subject to flooding or overly saturated ground, and where time and efficiency are not prioritized.[citation needed]

Because they are the engine that drives biological wastewater treatment, it is critical to closely monitor the quantity and quality of microorganisms in bioreactors. One method for this is via 2nd Generation ATP tests.

NASA tissue cloning bioreactor[edit]

In bioreactors in which the goal is to grow cells or tissues for experimental or therapeutic purposes, the design is significantly different from industrial bioreactors. Many cells and tissues, especially mammalian ones, must have a surface or other structural support in order to grow, and agitated environments are often destructive to these cell types and tissues. Higher organisms, being auxotrophic, also require highly specialized growth media.

NASA has developed a new type of bioreactor that artificially grows tissue in cell cultures. NASA's tissue bioreactor can grow heart tissue, skeletal tissue, ligaments, cancer tissue for study, and other types of tissue.[5]

For more information on artificial tissue culture, see tissue engineering.

See also[edit]

References[edit]

  1. ^ IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online corrected version:  (2006–) "bioreactor".
  2. ^ Lopez A, Lazaro N, Marques AM. The interphase technique: a simple method of cell immobilization in gel-beads. J Microbiol Methods, 1997, 30:231-234.
  3. ^ Peinado PA, Moreno JJ, Villaba JM, Gonzalez-Reyes JA, Ortega JM, Mauricio JC. A new immobilization method and their applications. Enzyme Microb Tech, 2006, 40:79-84.
  4. ^ Eva L. Decker und Ralf Reski (2008): Current achievements in the production of complex biopharmaceuticals with moss bioreactor. Bioprocess and Biosystems Engineering 31, 3-9 [1]
  5. ^ http://science.nasa.gov/NEWHOME/headlines/msad05oct99_1.htm

Further reading[edit]

External links[edit]