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Suggest pagesBis-tris propane
| Bis-tris propane | |
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2,2'-(Propane-1,3- |
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| Identifiers | |
| CAS number | 64431-96-5 |
| PubChem | 125132 |
| ChemSpider | 111383  |
| DrugBank | DB02676 |
| ChEBI | CHEBI:40947 |
| ChEMBL | CHEMBL63859 |
| Jmol-3D images | Image 1 Image 2 |
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| Properties | |
| Molecular formula | C11H26N2O6 |
| Molar mass | 282.34 g/mol |
| Melting point |
164-165 °C |
 (verify) (what is:  / ?)Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pKa values which are close in value. This buffer is primarily used in biochemistry and molecular biology.
[edit] Applications
A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR).[1] Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae.[2] It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodospin.[3] Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer.[4] The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane.[5]
[edit] See also
[edit] References
- ^ Eckert K.A., and Kunkel, T.A. (1991). "DNA polymerase fidelity and the polymerase chain reaction". PCR Methods Appl 1 (1): 17–24. PMID 1842916.
- ^ Song, L. (2003). "Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants". Anal. Biochem. 317 (2): 180–185. doi:10.1016/S0003-2697(03)00138-6. PMID 12758256.
- ^ Toth-Boconadi, R., et al. (2000). "Buffer effects on electric signals of light-excited bacteriorhodospin". Biophys. J. 78 (6): 3170–3177. doi:10.1016/S0006-3495(00)76853-6.
- ^ Bachouchi, N., (1986). "MgATPase activity of myosin subfragment 1. The dimer is more active than the monomer". J. Mol. Biol. 191 (2): 247–254. doi:10.1016/0022-2836(86)90261-5. PMID 2949083.
- ^ Wenner, J.R., and Bloomfield, V.A. (1999). "Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage". Anal. Biochem. 268 (2): 201–212. doi:10.1006/abio.1998.3079. PMID 10075809.
