Bisbenzimide

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Bisbenzimide
Bisbenzimide.svg
Names
IUPAC name
2’-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3’H-2,5’-bibenzimidazole
Other names
Hoechst 33342
Identifiers
ChemSpider 1420
Jmol-3D images Image
PubChem 1464
Properties
C27H28N6O
Molar mass 452.55 g·mol−1
Except where noted otherwise, data is given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
Infobox references

Bisbenzimide is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several bisbenzimides are used and are collectively called Hoechst stains.

Application[edit]

Bisbenzimides tend to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.

References[edit]