Bismarck brown Y

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Bismarck brown Y
Bismarck Brown Y.svg
CAS number 8005-77-4 N
PubChem 13981
ChemSpider 13374 N
Jmol-3D images Image 1
Image 2
Molecular formula C21H24N8
Molar mass 388.47 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Bismarck brown Y is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells. It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.

It was more common in the past; today it is partially replaced by other stains.

Bismarck brown Y is a constituent of Papanicolaou stains.

It can also be used as a counterstain for Victoria blue R for staining of acid-fast microorganisms.


Lillie, RD (1977) Conn's Biological Stains, 9th ed. Baltimore: Williams & Wilkins, p. 145-146.