Bismuth sulfite agar

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After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars).

Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. BLBG and bismuth stop gram-positive growth. Bismuth sulfite agar tests the ability to utilize ferrous sulfate and convert it to hydrogen sulfide.

Bismuth sulfite agar typically contains (w/v):[1]

1.6% bismuth sulfite Bi2(SO3)3
1.0% pancreatic digest of casein
1.0% pancreatic digest of animal tissue
1.0% beef extract
1.0% glucose
0.8% dibasic sodium phosphate
0.06% ferrous sulfate • 7 water
pH adjusted to 7.7 at 25 C

This medium is filter-sterilized, not autoclaved.

References[edit]

  1. ^ Atlas, R.M. (2004). Handbook of Microbiological Media. London: CRC Press. p. 68. ISBN 0-8493-1818-1.