In 2004, Lil Jon & The Eastside boyz recorded a song on "Bitches Ain't Shit" with Nate Dogg, Snoop Dogg, Oobie & Suga Free with similar lyrics to Dr. Dre's original, on the album Crunk Juice.
In 2005, Ben Folds released a cover version of "Bitches Ain't Shit", which reached number 71 on the Billboard Hot 100. Folds commonly includes the song in his live performances, one such time being on October 24, 2006, at his MySpace webcast gig. During his June 14, 2008 performance at the Bonnaroo Music and Arts Festival, Folds claimed he was retiring the song because people on the streets would call him, "...that 'Bitch' guy," in front of his kids. Two weeks later however he included the song in his set at the Glastonbury Festival. He claimed a friend had requested it to be played at her wedding. There are also a few a capella versions of Ben Folds' cover available on YouTube. In 2010, Folds took the song out of retirement and has played it as part of the encore at a concert in Indianapolis, Indiana, and Detroit, Michigan, as well as other concerts.
In 2010, rapper Lil Boosie made a remake titled "Niggas Ain't Shit", on his mixtape Gone Til' December.
In 2011 rapper YG sampled vocals from the song in his song "Bitches Ain't Shit" featuring Tyga and Nipsey Hussle.
In 2013 the creator of the Harlem Shake dance, George Miller, covered the beginning of the song on the ukulele as his alter ego "Pink Guy". It was later released in May 2014 as a track on his album Pink Guy.