BmKAEP (or anti-epilepsy peptide) is a neurotoxin from the venom of the Manchurian scorpion (Mesobuthus martensii). It is a β-toxin, which shift the activation voltage of sodium channels towards more negative potentials.
BmK is the abbreviation for Buthus martensi Karsch, an old name for the scorpion that is the source of BmKAEP; AEP is an abbreviation for anti-epilepsy peptide. At the NCBI Protein Database, the full name of this peptide is listed as "Toxin BmKAEP".
BmKAEP is a 61-amino-acid protein derived from an 85-amino-acid precursor. The mature protein contains 8 cysteine residues that establish 4 disulfide bridges (4C-C). Despite its high homology with other depressant toxins, BmKAEP differs from them at residues 6, 7 and 39, which is thought to be important in determining its unique function. Its lysine residue, at position 51, also has a special feature: it interacts with mammalian Na+ channels.
|BmKAEP primary sequence [Source: UniProt ]|
01 mklflllvis asmlidglvn adgyirgsng
Target and Mode of Action
Because of its sequence homology with other β-toxins, BmKAEP is predicted to bind to site 4 (S4) of voltage-gated Na+ channels, at domains I, III and IV. Its interaction with the S4 loop causes the loop to be maintained at the outward activated position. Therefore, activation of the Na+ channels shifts towards more negative values, enhancing the channel's activation and promoting spontaneous and repetitive firing. Subsequently, the sodium current amplitude decreases, due to the membrane potential depolarization, thus suppressing action potentials.
BmK venom induces a transient phase of contraction followed by a slow progressive flaccid paralysis in insect larvae. However, since it requires a high dosage to be effective, its toxicity is weak, both in insects and mammals.
|LD50||2,4 mg/kg (mice; intraperitoneal injection)|
|MLD (minimum lethal dose)||0,074 mg/kg (mice; Intracerebroventricular injection)|
|CPU (concentration paralysis unit)||1 µg/body (larvae)|
|NOAEL (No observed adverse effect)||<2 µg (insects); <20 µg (mice)|
Though the exact mechanism of its anti-epilepsy effect is not clear, several studies have shown that BmKAEP can inhibit coriaria lactone-induced epilepsy in rats by prolonging the latent epilepsy period, relieving the degree of seizures and shortening its average duration, at a pharmacological dosage of only 0,057 µg/g.
- "Toxin BmKAEP". NCBI Protein database. Retrieved 25 October 2010.
- Goudet, C; Chi, CW; Tytgat, J (2002). "An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch". Toxicon 40 (9): 1239–58. doi:10.1016/S0041-0101(02)00142-3. PMID 12220709.
- Wang, Chun-Guang; He, Xiao-Lin; Shao, Feng; Liu, Wei; Ling, Min-Hua; Wang, Da-Cheng; Chi, Cheng-Wu (2001). "Molecular characterization of an anti-epilepsy peptide from the scorpion Buthus martensi Karsch". European Journal of Biochemistry 268 (8): 2480–5. doi:10.1046/j.1432-1327.2001.02132.x. PMID 11298767.
- Yuan, Yuzhe; Luo, Lan; Peigneur, Steve; Tytgat, Jan; Zhu, Shunyi (2010). "Two recombinant depressant scorpion neurotoxins differentially affecting mammalian sodium channels". Toxicon 55 (8): 1425–33. doi:10.1016/j.toxicon.2010.02.019. PMID 20219516.
- Cestèle, S; Catterall, WA (2000). "Molecular mechanisms of neurotoxin action on voltage-gated sodium channels". Biochimie 82 (9–10): 883–92. doi:10.1016/S0300-9084(00)01174-3. PMID 11086218.
- Cestele, S; Qu, Y; Rogers, J; Rochat, H; Scheuer, T; Catterall, W (1998). "Voltage Sensor–TrappingEnhanced Activation of Sodium Channels by β-Scorpion Toxin Bound to the S3–S4 Loop in Domain II". Neuron 21 (4): 919–31. doi:10.1016/S0896-6273(00)80606-6. PMID 9808476.
- Gurevitz, Michael; Froy, Oren; Zilberberg, Noam; Turkov, Michael; Strugatsky, David; Gershburg, Eduard; Lee, Daewoo; Adams, Michael E. et al. (1998). "Sodium channel modifiers from scorpion venom: Structure–activity relationship, mode of action and application". Toxicon 36 (11): 1671–82. doi:10.1016/S0041-0101(98)00160-3. PMID 9792184.
- Zhou, XH; Yang, D; Zhang, JH; Liu, CM; Lei, KJ (1989). "Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion Buthus martensii Karsch". The Biochemical journal 257 (2): 509–17. PMC 1135608. PMID 2930463.
- NCBI Protein Database for "Toxin BmKAEP": AEP1_MESMA, ACCESSION: P15228.2, GI:37999913