Branched-chain alpha-keto acid dehydrogenase complex
The branched-chain α-ketoacid dehydrogenase complex (BCKDC) is a multi-subunit complex of enzymes that is found on the mitochondrial inner membrane. This enzyme complex catalyzes the oxidative decarboxylation of branched, short-chain alpha-ketoacids. BCKDC is a member of the mitochondrial α-ketoacid dehydrogenase complex family comprising pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, key enzymes that function in the Krebs cycle.
This complex requires the following 5 cofactors:
In animal tissue, BCKDC catalyzes an irreversible step in the catabolism of branched-chain amino acids—namely L-isoleucine, L-valine, and L-leucine and their derivatives (L-alpha-keto-beta-methylvalerate, alpha-ketoisovalerate, and alpha-ketoisocaproate, respectively). In bacteria, this enzyme participates in the synthesis of branched, long-chain fatty acids. In plants, this enzyme is involved in the synthesis of branched, long-chain hydrocarbons.
The overall catabolic reaction catalyzed by the BCKDC is shown in Figure 1.
The mechanism of enzymatic catalysis by the BCKDC draws largely upon the elaborate structure of this large enzyme complex. This enzyme complex is composed of three catalytic components: alpha-ketoacid dehydrogenase (also referred to as the E1 component), dihydrolipoyl transacylase (E2 component), and dihydrolipoamide dehydrogenase (E3 component). In humans, 24 copies of E2 arranged in octahedral symmetry form the core of the BCKDC. Non-covalently linked to this polymer of 24 E2 subunits are 12 E1 α2β2 tetramers and 6 E3 homodimers. In addition to the E1/E3-binding domain, there are 2 other important structural domains in the E2 subunit: (i) a lipoyl-bearing domain in the amino-terminal portion of the protein and (ii) an inner-core domain in the carboxy-terminal portion. The inner-core domain is linked to the other two domains of the E2 subunit by two interdomain segments (linkers). The inner-core domain is necessary to form the oligomeric core of the enzyme complex and catalyzes the acyltransferase reaction (shown in the "Mechanism" section below). The lipoyl domain of E2 is free to swing between the active sites of the E1, E2, and E3 subunits on the assembled BCKDC by virtue of the conformational flexibility of the aforementioned linkers (see Figure 2). Thus, in terms of function as well as structure, the E2 component plays a central role in the overall reaction catalyzed by the BCKDC.
The role of each subunit is as follows:
E1 uses thiamine pyrophosphate (TPP) as a catalytic cofactor. E1 catalyzes both the decarboxylation of the α-ketoacid and the subsequent reductive acylation of the lipoyl moiety (another catalytic cofactor) that is covalently bound to E2.
E2 catalyzes a transfer of the acyl group from the lipoyl moiety to coenzyme A (a stoichiometric cofactor).
The E3 component is a flavoprotein, and it re-oxidizes the reduced lipoyl sulfur residues of E2 using FAD (a catalytic cofactor) as the oxidant. FAD then transfers these protons and electrons to NAD+ (a stoichiometric cofactor) to complete the reaction cycle.
As previously mentioned, BCKDC’s primary function in mammals is to catalyze an irreversible step in the catabolism of branched-chain amino acids. However, the BCKDC has a relatively broad specificity, also oxidizing 4-methylthio-2-oxobutyrate and 2-oxobutyrate at comparable rates and with similar Km values as for its branched-chain amino acid substrates. The BCKDC will also oxidize pyruvate, but at such a slow rate this side reaction has very little physiological significance.
The reaction mechanism is as follows. Please note that any of several branched-chain α-ketoacids could have been used as a starting material; for this example, α-ketoisovalerate was arbitrarily chosen as the BCKDC substrate.
- NOTE: Steps 1 and 2 occur in the E1 domain
STEP 1: α-ketoisovalerate combines with TPP and is then decarboxylated. The proper arrow-pushing mechanism is shown in Figure 3.
STEP 2: The 2-methylpropanol-TPP is oxidized to form an acyl group while being simultaneously transferred to the lipoyl cofactor on E2. Note that TPP is regenerated. The proper arrow-pushing mechanism is shown in Figure 4.
- NOTE: The acylated lipoyl arm now leaves E1 and swings into the E2 active site, where Step 3 occurs.
STEP 3: Acyl group transfer to CoA. The proper arrow-pushing mechanism is shown in Figure 5.
- *NOTE: The reduced lipoyl arm now swings into the E3 active site, where Steps 4 and 5 occur.
STEP 4: Oxidation of the lipoyl moiety by the FAD coenzyme, as shown in Figure 6.
STEP 5: Reoxidation of FADH2 to FAD, producing NADH:
- FADH2 + NAD+ --> FAD + NADH + H+
A deficiency in any of the enzymes of this complex as well as an inhibition of the complex as a whole leads to a buildup of branched-chain amino acids and their harmful derivatives in the body. These accumulations lend a sweet smell to bodily excretions (such as ear wax and urine), leading to a pathology known as maple syrup urine disease.
This enzyme is an autoantigen recognized in primary biliary cirrhosis, a form of acute liver failure. These antibodies appear to recognize oxidized protein that has resulted from inflammatory immune responses. Some of these inflammatory responses are explained by gluten sensitivity. Other mitochondrial autoantigens include pyruvate dehydrogenase and branched-chain oxoglutarate dehydrogenase, which are antigens recognized by anti-mitochondrial antibodies.
- Indo I, Kitano A, Endo F, Akaboshi I, Matsuda, I (1987). "Altered Kinetic Properties of the Branched-Chain Alpha-Keto Acid Dehydrogenase Complex Due to Mutation of the Beta-Subunit of the Branched-Chain Alpha-Keto Acid Decarboxylase (E1) Component in Lymphoblastoid Cells Derived from Patients with Maple Syrup Urine Disease". J Clin Invest 80 (1): 63–70. doi:10.1172/JCI113064. PMID 3597778.
- Yeaman SJ (1989). "The 2-oxo acid dehydrogenase complexes: recent advances.". Biochem J. 257 (3): 625–632. PMID 2649080.
- Broquist HP, Trupin JS. (1966). "Amino Acid Metabolism". Annual Review of Biochemistry 35: 231–247. doi:10.1146/annurev.bi.35.070166.001311.
- Harris RA, Paxton R, Powell SM, Goodwin GW, Kuntz MJ, Han AC. (1986). "Regulation of branched-chain alpha-ketoacid dehydrogenase complex by covalent modification.". Adv Enzyme Regul. 25: 219–237. doi:10.1016/0065-2571(86)90016-6. PMID 3028049.
- Namba Y, Yoshizawa K, Ejima A, Hayashi T, Kaneda T. (1969). "Coenzyme A- and nicotinamide adenine dinucleotide-dependent branched chain alpha-keto acid dehydrogenase. I. Purification and properties of the enzyme from Bacillus subtilis.". J Biol Chem. 244 (16): 4437–4447. PMID 4308861.
- Lennarz WJ et al. (1961). "The role of isoleucine in the biosynthesis of branched-chain fatty acids by micrococcus lysodeikticus.". Biochemical and Biophysical Research Communications 6 (2): 1112–116. doi:10.1016/0006-291X(61)90395-3. PMID 14463994.
- Aevarsson A, Chuang JL, Wynn RM, Turley S, Chuang DT, Hol WGJ. (2000). "Crystal structure of human branched-chain α-ketoacid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease". Structure 8 (3): 277–291. doi:10.1016/S0969-2126(00)00105-2. PMID 10745006.
- Chuang DT. (1989). "Molecular studies of mammalian branched-chain alpha-keto acid dehydrogenase complexes: domain structures, expression, and inborn errors.". Annals of the New York Academy of Sciences 573: 137–154. doi:10.1111/j.1749-6632.1989.tb14992.x. PMID 2699394.
- Chuang DT, Hu CW, Ku LS, Markovitz PJ, Cox RP. (1985). "Subunit structure of the dihydrolipoyl transacylase component of branched-chain alpha-keto acid dehydrogenase complex from bovine liver. Characterization of the inner transacylase core.". J Biol Chem 260 (25): 13779–86. PMID 4055756.
- Reed LJ, Hackert ML. (1990). "Structure-function relationships in dihydrolipoamide acyltransferases.". J Biol Chem 265 (16): 8971–8974. PMID 2188967.
- Perham RN. (1991). "Domains, motifs, and linkers in 2-oxo acid dehydrogenase multienzyme complexes: a paradigm in the design of a multifunctional protein.". Biochemistry 30 (35): 8501–8512. doi:10.1021/bi00099a001. PMID 1888719.
- Berg, Jeremy M., John L. Tymoczko, Lubert Stryer, and Lubert Stryer. Biochemistry. 6th ed. New York: W.H. Freeman, 2007. 481. Print.
- Heffelfinger SC, Sewell ET, Danner DJ. (1983). "Identification of specific subunits of highly purified bovine liver branched-chain ketoacid dehydrogenase.". Biochemistry 22 (24): 5519–5522. doi:10.1021/bi00293a011. PMID 6652074.
- Jones SM, Yeaman SJ. (1986). "Oxidative decarboxylation of 4-methylthio-2-oxobutyrate by branched-chain 2-oxo acid dehydrogenase complex.". Biochemical Journal 237 (2): 621–623. PMC 1147032. PMID 3800905.
- Pettit FH, Yeaman SJ, Reed LJ. (1978). "Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney.". Proceedings of the National Academy of Sciences of the United States of America 75 (10): 4881–4885. doi:10.1073/pnas.75.10.4881. PMC 336225. PMID 283398.
- Damuni Z, Merryfield ML, Humphreys JS, Reed LJ. (1984). "Purification and properties of branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney.". Proceedings of the National Academy of Sciences of the United States of America 81 (14): 4335–4338. doi:10.1073/pnas.81.14.4335. PMC 345583. PMID 6589597.
- Berg, Jeremy M., John L. Tymoczko, Lubert Stryer, and Lubert Stryer. Biochemistry. 6th ed. New York: W.H. Freeman, 2007. 478-79. Print.
- Podebrad F, Heil M, Reichert S, Mosandl A, Sewell AC, Böhles H (April 1999). "4,5-dimethyl-3-hydroxy-25H-furanone (sotolone)--the odour of maple syrup urine disease". Journal of Inherited Metabolic Disease 22 (2): 107–114. doi:10.1023/A:1005433516026. PMID 10234605.
- Leung PS, Rossaro L, Davis PA et al. (2007). "Antimitochondrial antibodies in acute liver failure: Implications for primary biliary cirrhosis". Hepatology 46 (5): 1436–42. doi:10.1002/hep.21828. PMC 3731127. PMID 17657817.
- GeneReviews/NCBI/NIH/UW entry on Maple Syrup Urine Disease
- Branched Chain Ketoacid Dehydrogenase at the US National Library of Medicine Medical Subject Headings (MeSH)
- EC 184.108.40.206